双模板分子印迹聚合物用于同时检测马铃薯中的激动素和6-苄氨基嘌呤

Dual-template molecularly imprinted polymers for simultaneous detection of Kinetin and 6-Benzylaminopurine in potatoes

以激动素(KT)和6-苄氨基嘌呤(BAP)2种植物生长调节剂为模板,甲基丙烯酸为功能单体,制备双模板分子印迹聚合物(DMIP)。通过静态和动力学吸附实验评估了其对KT和BAP的分子识别能力和结合能力,发现与单模板MIP和非印迹聚合物(NIP)相比,DMIP对2种模板都表现出更好的亲和力和选择性。DMIP对KT和BAP的最大吸附容量分别为6.39和5.64μmol/g,印迹因子IF_(KT)和IF_(BAP)分别为4.26和3.97,在40 min时达到吸附平衡。建立并优化了基于分子印迹固相萃取(MISPE)和高效液相色谱(HPLC)法的马铃薯中KT和BAP的检测方法。在加标浓度为0.05,0.1和0.15 mmol/L时,本方法检测KT和BAP的回收率分别为95.3%~100%和94.2%~100%,相对标准偏差(RSDs)小于2.3%。所建立的方法适用于马铃薯中KT和BAP的定量检测。

Dual-template molecularly imprinted polymers(DMIP)were synthesized using Kinetin(KT)and 6-Benzylaminopurine(BAP)as templates and methacrylic acid as the functional monomer.The molecular recognition ability and the binding capability of the as-prepared polymers towards KT and BAP were evaluated via static and dynamic binding tests,and it was found that the DMIP showed better affinity and selectivity for both templates compared with single-templated MIP and non-imprinted polymer(NIP).The maximum adsorption capacities of DMIP for KT and BAP were 6.39 and 5.64μmol/g,imprinting factors IF_(KT) and IF_(BAP) were 4.26 and 3.97,respectively,and the adsorption equilibrium reached at 40 min.A rapid and accurate determination strategy was established for the simultaneous detection of KT and BAP directly in potatoes by coupling molecularly imprinted solid-phase extraction(MISPE)with high performance liquid chromatography(HPLC).When the spiked concentrations were 0.05,0.1,and 0.15 mmol/L,the recovery rates for KT and BAP were 95.3%-100%and 94.2%-100%,respectively,with the relative standard deviations(RSDs)less than 2.3%.This method is suitable for the quantitative detection of KT and BAP in potatoes.