分子印迹前处理结合光纤传感-微顺序注射-阀上实验室在线衍生化检测血浆中的异丙酚

Development of a micro sequential injection lab-on-valve system combined with molecularly imprinted pretreatment and fiber optic sensing for the online derivatization and determination of propofol in plasma

将分子印迹、在线衍生化与微顺序注射-光纤传感技术相结合,建立了在线前处理、在线检测、自动化分析的异丙酚光纤传感分析系统和方法。通过本体聚合法合成异丙酚分子印迹聚合物,构建异丙酚分子印迹固相萃取在线前处理系统,通过阀切换模式完成样品的预处理和分析检测的切换,实现在线前处理。通过光纤传感-微顺序注射-阀上实验室检测系统进行异丙酚衍生化反应和衍生化产物的光纤传感检测,使异丙酚分析过程一体化、自动化。该检测方法的线性范围为1.0~15.0μg/mL,线性相关系数r=0.9982,定量限为1.0μg/mL,加样回收率为101.7%~111.4%,相对标准偏差(RSD)小于5%。该方法为麻醉药物异丙酚血药浓度监测提供了检测新方法。

The molecular imprinting technology,on-line chemical derivatization,micro sequential injection analysis technology and fiber optic sensing technology were combined to establish a propofol fiber optic sensing analysis system and analysis method for online pretreatment,online detection and automated analysis of propofol.Propofol molecularly imprinted polymers were synthesized by bulk polymerization.The online pretreatment system for molecularly imprinted solid phase extraction of propofol was established.The pretreatment and the detection of the sample were switched by valve switching technique.Through combining the optical fiber sensing with micro sequential injection lab-on-valve analysis system,the propofol derivatization reaction was carried out,and the derivative products were detected by the optical fiber sensing,which promoted the integration and automation of the propofol analysis process.The linear range of this method was 1.0-15.0μg/mL,the correlation coefficient(r)was 0.9982,the limit of quantification was 1.0μg/mL,the recoveries were 101.7%-111.4%,and the relative standard deviation(RSD)was less than 5%.Thus,a new method for monitoring the plasma concentration of anesthetic propofol was provided.