PEDV感染导致宿主脂质代谢变化

PEDV infection alters host lipid metabolism

为了探究猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)感染后宿主的脂质代谢变化,将PEDV感染仔猪及Vero细胞后,收集血液、肠道及细胞样本,并通过血液生化分析、流式细胞术和免疫荧光法检测宿主甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)含量,通过qPCR检测宿主脂代谢相关酶的基因转录水平变化情况。结果显示:PEDV感染显著提升了宿主总脂质水平,且显著促进了仔猪肠道及Vero细胞脂肪酸和胆固醇代谢相关酶的表达。综上,PEDV感染导致宿主总脂质水平上升,仔猪肠道及Vero细胞脂质代谢重塑,脂代谢基因变化,该研究结果表明,脂代谢相关基因可作为影响PEDV复制的关键靶点,为后续其防控和治疗提供新的理论依据。

In order to investigate the changes in lipid metabolism of the host after infection with porcine epidemic diarrhea virus(PEDV),blood,intestinal and cellular samples were collected from PEDV-infected piglets and Vero cells,and the levels of triglyceride(TG)and total cholesterol(TC)were detected by blood biochemical analyses,flow cytometry,and immunofluorescence,and changes in the gene levels of the enzymes related to lipid metabolism of the hosts were detected by qPCR.The results showed that PEDV infection significantly elevated host total lipid levels and significantly promoted the expression of enzymes related to fatty acid and cholesterol metabolism in the intestine and Vero cells of piglets.In conclusion,PEDV infection leads to an increase in host total lipid levels,remodeling of lipid metabolism in piglets intestine and Vero cells,and lipid metabolism gene changes.The results of this study show that lipid metabolism-related genes can be used as a key target to influence PEDV replication,which will provide a new theoretical basis for the subsequent prevention,control and treatment of PEDV infection.