膝骨关节炎不同证候患者关节液白介素-1β浓度特点研究

Study on the Characteristics of Interleukin-1βConcentration in Synovial Fluid of Patients with Knee Osteoarthritis with Different Syndromes

目的:探讨膝骨关节炎痰瘀互结证和肝肾亏虚证两种不同证候患者关节液白介素-1β(IL-1β)的浓度特点,并分析其与证候评分之间的相关性。方法:共纳入79例膝骨关节炎患者作为研究对象,根据中医证候诊断标准将患者分为痰瘀互结证组和肝肾亏虚证组,通过收集分析两组患者关节液样本,比较两组患者之间的IL-1β浓度差异,对关节液IL-1β浓度特点及其中医证候评分进行相关性分析。结果:两组患者的IL-1β浓度水平有显著差异,其中痰瘀互结证IL-1β浓度水平高于肝肾亏虚证,差异有统计学意义(P<0.05);痰瘀互结证IL-1β浓度水平与中医证候评分正相关,差异有统计学意义(r=0.389,P<0.05);肝肾亏虚证IL-1β浓度水平与中医证候评分无相关性,差异无统计学意义(r=0.146,P>0.05)。结论:膝骨关节炎不同证候患者IL-1β的浓度特点不同,痰瘀互结证IL-1β的浓度水平可以在一定程度上反映证候的严重程度,提示痰瘀互结证和肝肾亏虚证炎症因子的浓度差异可能反映不同的证候特点。

Objective:To study the characteristics of interleukin-1β(IL-1β)concentration in individuals with two distinct knee osteoarthritis syndromes,and the relationship between the syndrome score and the features of IL-1βconcentration.Methods:79 patients in all with knee osteoarthritis were enrolled as the subjects.The patients were divided into two groups:stagnation of phlegm and blood stasis syndrome group and liver and kidney deficiency syndrome group according to the diagnostic criteria of traditional Chinese medicine(TCM)syndromes.The joint fluid samples of the two groups were collected and analyzed to compare the difference of IL-1βconcentration in two groups,and the correlation between the concentration characteristics of IL-1βin synovial fluid and the score of TCM syndrome was analyzed.Results:The concentration of IL-1βvaried significantly between the two groups,in patients with stagnation of phlegm and blood stasis syndrome,the concentration was higher than that in liver and kidney deficiency syndrome(P<0.05).The correlation between the IL-1βconcentration in stagnation of phlegm and blood stasis syndrome and the syndrome score was positive and statistically significant(r=0.389,P<0.05),and there was no correlation between the syndrome score and the IL-1βconcentration in liver and kidney deciency syndrome(r=0.146,P>0.05).Conclusion:The concentration features of IL-1βin synovial fluid vary across patients suffering from different syndromes.Patients with blood stasis and stagnation of phlegm syndrome may have varying concentrations of IL-1β,which may indicate a correlation between the severity of the syndrome and the characteristics of the various inflammatory factor concentrations among patients with osteoarthritis of the knee.