兔源大肠杆菌、奇异变形杆菌和球虫混合感染及药物敏感性分析

Mixed Infection and Drug Sensitivity Analysis of Escherichia coli,Proteus mirabilis and Coccidia from Rabbits

旨在确定引起河南某规模化兔场病死兔发病的病原及其药物敏感性。本研究对河南某规模化兔场中病死兔进行剖检并采集3份病死兔病料组织,通过问诊,结合发病情况、临床表现、剖检变化、细菌的分离培养、生化试验、16S rRNA PCR鉴定、构建系统进化树和药敏试验,确定引起该兔场发病的病原及其药物敏感性。结果显示,经细菌分离培养得到2株大小不等的革兰阴性短杆菌(分别命名为LY-01和LY-02株),其分别符合大肠杆菌和奇异变形杆菌的生化特性。经PCR鉴定发现,LY-01和LY-02的16S rRNA基因序列片段大小分别为833 bp和1500 bp。经同源比对分析发现LY-01与OR717622.1同源性为99.88%;LY-02与CP042907.1同源性为99.93%。经球虫检查发现,其粪便中存在大量球虫卵,其OPG值分别为0.64×10^(4)、0.62×10^(4)和0.68×10^(4),均达到肠球虫感染标准。药敏试验结果显示,分离得到的大肠杆菌和奇异变形杆菌同时对青霉素和头孢曲松敏感。综上,本研究为家兔养殖过程中大肠杆菌、奇异变形杆菌和球虫混合感染的防治提供了理论依据。

The purpose of this study was to determine the pathogen and its drug sensitivity of rabbits who died in a large-scale rabbit farm in Henan province.In this study,the dead rabbits in a large-scale rabbit farm in Henan province were dissected,three rabbits’diseased tissues were collected,the pathogen causing the disease in the rabbit farm and its drug sensitivity were determined by asking questions,combining with the incidence,clinical manifestations,autopsy changes,bacterial isolation and culture,biochemical tests,16S rRNA PCR identification,phylogenetic tree construction and drug sensitivity tests.The results showed that two strains of Gram-negative Brevibacterium(named LY-01 and LY-02,respectively)with different sizes were obtained by bacterial isolation and culture,which accorded with the biochemical characteristics of Escherichia coli and Proteus mirabilis,respectively.It was found by PCR that the 16S rRNA gene fragments of LY-01 and LY-02 were 833 bp and 1500 bp,respectively.The homology of LY-01 and OR717622.1 was 99.88%.The homology of LY-02 and CP042907.1 was 99.93%.After coccidiosis examination,it was found that there were a large number of coccidia eggs in their feces,and their OPG values were 0.64×10^(4),0.62×10^(4)and 0.68×10^(4),respectively,which all reached the infection standard of enterococcidia.The results of drug sensitivity test showed that Escherichia coli and Proteus mirabilis were sensitive to both penicillin and ceftriaxone.To sum up,this study provides a theoretical basis for the prevention and treatment of mixed infection of Escherichia coli,Proteus mirabilis and coccidia in rabbit breeding.