渗透压调节基因在入侵物种红耳龟适应盐度环境中的作用

Role of Osmotic Pressure Regulating Genes in the Adaptation of Invasive Species Trachemys scripta elegans to Salinity Environments

为了解入侵物种红耳龟Trachemys scripta elegans在盐度水域中的渗透压调节能力,本研究在盐度5‰和15‰(S5组和S15组)的环境中对红耳龟进行了24 h、48 h胁迫,应用实时荧光定量PCR技术检测渗透压调节相关基因的mRNA相对表达量。红耳龟在2个盐度处理下24 h时,肾脏中水通道蛋白1(AQP1)、热休克蛋白(HSP70)、钠氯协同转运蛋白(NCC)、钠钾氯协同转运蛋白(NKCC)、肌醇转运蛋白(SMIT)和牛磺酸转运蛋白(TAUT)mRNA的相对表达量显著提高,与对照组的差异显著(P<0.05);胁迫48 h时,HSP70、NCC、NKCC和TAUT mRNA的相对表达量显著高于对照组(P<0.05),但S15组AQP1和SMIT mRNA的变化不显著。红耳龟在适应半咸水环境过程中,渗透压调节基因发挥了重要的调节作用,揭示了红耳龟可能采用特定的渗透压调节策略。研究结果有助于深入认识入侵物种的生理适应机制,为管理者制定科学的管理措施提供数据支持,以控制或减少红耳龟的负面生态影响。

In order to understand the osmotic pressure regulation ability of invasive species(Trachemys scripta elegans)in saline waters,T.scripta elegans were fed in salinity(5‰and 15‰,groups S5 and S15)environments for 24 h and 48 h.Real-time fluorescence quantitative PCR was used to detect the relative mRNA expression of genes related to osmotic pres-sure regulation.The results showed that compared with the control group,the mRNA relative expression levels of AQP1,HSP70,NCC,NKCC,SMIT and TAUT in the kidney tissue of the treatment group were significantly(P<0.05)increased after being exposed to salinity of 5‰and 15‰for 24 h.After 48 h of salinity stress,the mRNA relative expression levels of HSP70,NCC,NKCC and TAUT were significantly higher than those of the control group(P<0.05),while the expres-sion of AQP1 and SMIT in S15 group were not significantly changed.This indicated that the osmotic pressure regulation genes might play an important role in the adaptation of T.scripta elegans to brackish water environment.Further under-standing the physiological adaptation mechanisms of invasive species may contribute to controlling or reducing the negative ecological impact of T.scripta elegans by developing scientific management measures.