STING agonist 22免疫增强剂配伍ASFV P30蛋白对猪免疫效果的评价

Evaluation of the immune efficacy of STING agonist 22 immune enhancer combined with ASFV P30 protein in pigs

本试验旨在探究STING agonist 22为候选免疫增强剂,配伍ASFV P30靶标蛋白开展对猪的免疫效果评价,为非洲猪瘟亚单位疫苗免疫增强剂的筛选提供前期实验依据。选取本实验室原核表达ASFV免疫原性较高的P30结构蛋白作为靶标抗原,并结合STINGagonist 22免疫增强剂与ISA201VG佐剂配伍ASFV P30蛋白2次免疫试验猪,比较STING agonist 22免疫增强剂诱导细胞免疫、体液免疫和抗原递呈反应的增强作用。结果显示,与单独201VG佐剂配伍相比,增加STING agonist 22免疫增强剂组免疫试验猪可以显著提早P30介导的抗体应答时间,提高B细胞成熟分化相关转录因子的表达水平,促进DCs成熟并增强其抗原递呈。本研究结果表明,STING agonist 22免疫增强剂配伍ASFV P30蛋白可以明显提高猪的细胞免疫、体液免疫反应及诱导更强的抗原递呈,可作为一种ASF亚单位疫苗的免疫增强剂。

This study evaluated the immune effect of ASFV P30 target protein in pigs using STING agonist 22 as a candidate immune enhancer,and provided a preliminary experimental basis for screening the immunity booster of African swine fever(ASF)subunit vaccine.The P30 structural protein with high immunogenicity of prokaryotic expression of ASFV in our laboratory was selected as the target antigen and combined with STING agonist 22 immune enhancer and ISA 201VG adjuvant dosed with ASFV P30 protein for 2 immunization test pigs to compare the enhancement effect of STING agonist 22 immune enhancer in inducing cellular immunity,humoral immunity,and antigen delivery response.The results showed that the addition of STING agonist 22 immune enhancer group immunized test pigs significantly advanced the time of P30-mediated antibody response,increased the expression level of B cell maturation and differentiation-related transcription factors,and promoted the maturation of DCs and enhanced their antigen delivery,compared with 201VG adjuvant dosing alone.The results of this study showed that STING agonist 22 immune enhancer paired with ASFV P30 protein could significantly enhance cellular and humoral immune responses and induce stronger antigen presentation in pigs,and could be used as an immune enhancer for ASF subunit vaccines.