小檗碱体内代谢产物对前脂肪细胞分化和脂代谢的影响

Effects of berberine metabolites on differentiation and lipid metabolism of preadipocytes

目的探究小檗碱(BBR)体内代谢产物对3T3-L1前脂肪细胞分化和脂代谢的作用。方法将3T3-L1前脂肪细胞分为对照组和BBR代谢产物处理组,用细胞增殖计数法检测细胞存活率。BBR、二氢小檗碱(DHB)、小檗红碱(BRB)、非洲防己碱(COL)、巴马汀(PAL)、药根碱(JAT)和去亚甲基小檗碱(DEM)组分别给予5μmol·L^(-1)BBR、2.5μmol·L^(-1)DHB、10μmol·L^(-1)BRB、20μmol·L^(-1)COL、25μmol·L^(-1)PAL、50μmol·L^(-1)JAT和50μmol·L^(-1)DEM处理;对照组给予正常培养。用实时荧光定量聚合酶链反应法检测脂蛋白脂肪酶(LPL)、乙酰辅酶A羧化酶A(ACACA)等mRNA的表达水平。结果DHB、COL、PAL、JAT、DEM孵育48 h与对照组相比,均不影响细胞存活率,20μmol·L^(-1)BRB可将细胞活性降至(86.55±1.99)%。培养12 d后,对照组和BBR、DHB、BRB、COL、PAL、JAT、DEM组的LPL mRNA相对表达水平分别为1.00±0.02、0.41±0.04、0.44±0.04、1.40±0.08、1.27±0.10、1.15±0.07、3.06±0.30和1.45±0.07,ACACA mRNA相对表达水平分别为1.01±0.01、0.62±0.04、0.69±0.06、1.78±0.17、4.77±0.19、4.20±0.14、6.38±0.30和1.18±0.08。BBR、DHB、JAT组的LPL mRNA相对表达水平与对照组比较,BRB、COL、PAL、JAT组的ACACA mRNA相对表达水平与对照组比较,在统计学上差异均有统计学意义(均P<0.05)。结论小檗碱体内代谢产物可以抑制脂肪细胞分化和促进脂代谢,从而减少细胞中脂质蓄积。

Objective To investigate the effect of berberine(BBR)metabolites on 3T3-L1 preadipocytes differentiation and lipid metabolism.Methods 3T3-L1 preadipocytes were divided into control group and BBR metabolites treated-group.The cell viability was measured by cell proliferation counting method.The BBR,dihydroberberine(DHB),berberrubine(BRB),columbamine(COL),palmatine(PAL),jatrorrhizine(JAT)and demethyleneberberine(DEM)groups were treated with 5μmol·L^(-1) BBR,2.5μmol·L^(-1) DHB,10μmol·L^(-1) BRB,20μmol·L^(-1) COL,25μmol·L^(-1) PAL,50μmol·L^(-1) JAT and 50μmol·L^(-1) DEM,respectively;the control group was cultured normally.After inducing differentiation,expression levels of lipoprotein lipase(LPL),and acetyl-CoA carboxylase A(ACACA)were detected by real-time fluorescence quantitative polymerase chain reaction.Results Compared with the control group,the DHB,COL,PAL,JAT and DEM did not affect the cell viability when inc ub ated at the used concentration for 48 h.However,20μmol·L^(-1) BRB significantly decreased the cell viability to(86.55±1.99)%.Twelve days after treatment,the relative expression levels of LPL mRNA in control,BBR,DHB,BRB,COL,PAL,JAT and DEM groups were 1.00±0.02,0.41±0.04,0.44±0.04,1.40±0.08,1.27±0.10,1.15±0.07,3.06±0.30 and 1.45±0.07;the relative expression levels of ACACA mRNA were 1.01±0.01,0.62±0.04,0.69±0.06,1.78±0.17,4.77±0.19,4.20±0.14,6.38±0.30 and 1.18±0.08,respectively.The relative expression levels of LPL in BBR,DHB and JAT groups and the relative expression levels of ACACA in BRB,COL,PAL and JAT groups were all compared with those of the control group,and the differences were statistically significant(all P<0.05).Conclusion BBR metabolites can inhibit differentiation and promote lipid metabolism in adipocytes,which therefore reduce lipid accumulation.