非小细胞肺癌患者miR-221、miR-630表达及与预后的关系分析

Analysis of the expressions of miR-221 and miR-630 and their relationship with prognosis in patients with non-small cell lung cancer

目的分析非小细胞肺癌(NSCLC)miR-221、miR-630表达及与预后的关系。方法采集198例NSCLC患者癌组织及癌旁组织标本,检测miR-221、miR-630表达,分析与病理特征关系,Kaplan-Meier法分析2年生存情况,Cox回归分析miR-221、miR-630表达与预后的关系。结果癌组织miR-221表达高于癌旁组织,miR-630表达低于癌旁组织(P<0.05)。TNM分期Ⅲ~Ⅳ期、有淋巴结转移的NSCLC患者肿瘤组织miR-221表达高于TNM分期Ⅰ~Ⅱ期、无淋巴结转移患者(P<0.05);TNM分期Ⅲ~Ⅳ期、肿瘤直径≥4 cm、有淋巴结转移的NSCLC患者肿瘤组织miR-630表达低于TNM分期Ⅰ~Ⅱ期、肿瘤直径<4 cm、无淋巴结转移患者(P<0.05)。Kaplan-Meier分析显示,miR-221高表达者2年生存率为55.56%,低于miR-221低表达者的79.63%;miR-630高表达者2年生存率为75.96%,高于miR-630低表达的50.00%(P<0.05)。Cox回归分析显示,TNM分期、淋巴结转移及miR-221、miR-630表达是NSCLC预后的影响因素(P<0.05)。Spearman相关分析显示,miR-221与miR-630表达呈负相关性(P<0.05)。结论NSCLC患者miR-221表达升高,miR-630表达降低,且与TNM分期、淋巴结转移和预后相关,可作为NSCLC患者潜在预后评估的标记物。

Objective To analyze the expressions of microRNA-221(miR-221)and microRNA-630(miR-630)in patients with non-small cell lung cancer(NSCLC)and their relationship with the prognosis.Methods Cancer tissue and adjacent tissue samples from 198 patients with NSCLC were collected.The expressions of miR-221 and miR-630 were detected.The relationship with pathological characteristics was analyzed.Kaplan-Meier method was used to analyze 2-year survival.Cox regression analysis was conducted to investigate the relationship between the expressions of miR-221 and miR-630 and the prognosis.Results The expressions of miR-221 in cancer tissues was higher than that in adjacent tissues,and the expressions of miR-630 was lower than that in adjacent tissues(P<0.05).The expression of miR-221 in tumor tissue of NSCLC patients at TNM stage III-IV with lymph node metastasis was higher than that in patients at TNM stage I-II and without lymph node metastasis(P<0.05).The expression of miR-630 in tumor tissues of NSCLC patients at TNM stage III-IV with tumor diameter≥4 cm and with lymph node metastasis was lower than that in patients at TNM stage I-II with tumor diameter<4 cm and without lymph node metastasis(P<0.05).Kaplan-Meier analysis showed that the 2-year survival rate of those with high expression of miR-221 was 55.56%that was lower than 79.63%of those with low expression of miR-221.The 2-year survival rate of those with high expression of miR-630 was 75.96%that was higher than 50.00%of those with low expression of miR-630(P<0.05).Cox regression analysis showed that TNM stage,lymph node metastasis,and expressions of miR-221 and miR-630 were prognostic factors of NSCLC(P<0.05).Spearman correlation analysis showed that the expression of miR-221 was negatively correlated with that of miR-630(P<0.05).Conclusions The expression of miR-221 is increased and expression of miR-630 is decreased in patients with NSCLC.Their expressions are related to TNM staging,lymph node metastasis,and the prognosis.The two can be used as potential prognostic markers in patients with NSCLC.