黄精醇提物佐剂对小鼠免疫效果的评价

Evaluation of the immunizing effect of adjuvant of alcohol extractof Polygonatum sibiricum F. on mice

为了评价黄精醇提物佐剂对小鼠的免疫效果,将60只ICR小鼠随机分为12组,即空白组、模型组、对照组、PSE30组(低、中、高)、PSE50组(低、中、高)和PSE70组(低、中、高),定期接种OVA,检测免疫细胞数量及活性、血清特异性抗体水平、脾细胞增殖及分泌IL-2含量。结果显示:与模型组相比,PSE30中、高剂量组、PSE50高剂量组和PSE70高剂量组的NK细胞活性显著提高(P<0.05),PSE50中、高剂量组小鼠血清IgG、IgG1、IgG2a和IgG2b滴度显著升高(P<0.05);PSE50高剂量组和PSE70低、高剂量组均能促进小鼠脾细胞增殖(P<0.05);PSE50中、高剂量组和PSE70高剂量组小鼠脾细胞分泌的IL-2含量显著升高(P<0.05);对照组的中性粒细胞数量、NK细胞活性、IgG、IgG1、IgG2a和IgG2b滴度、脾细胞增殖能力及分泌的IL-2含量均极显著升高(P<0.01)。综上,黄精醇提物佐剂可显著提高小鼠的细胞免疫和体液免疫功能,以PSE50中、高剂量组效果最佳。

In order to evaluate the immunization effect of alcohol extract of Polygonatum sibiricum F.adjuvant in mice,60 ICR mice were randomly divided into 12 groups,i.e.,blank group,model group,control group,PSE30 group(low,medium and high),PSE50 group(low,medium and high),PSE70 group(low,medium and high),which were regularly inoculated with OVA to detect the number and activity of immune cells,serum specific antibody levels,proliferative capacity of splenocytes and the content of secreted IL-2.The results showed that compared with the model group,NK cell activity was significantly increased in the medium and high dose groups of PSE30,the high dose group of PSE50,and the high dose group of PSE70(P<0.05);the antibody titers of IgG,IgG1,IgG2a and IgG2b of mice in the medium and high dose groups of PSE50 were significantly elevated(P<0.05);the high dose group of PSE50 and the low,high dose groups of PSE70all promoted the proliferation of mouse splenocytes(P<0.05);the content of IL-2 secreted by mouse splenocytes in the middle and high dose groups of PSE50 and the high dose group of PSE70 was significantly elevated(P<0.05);in the control group,the number of neutrophils,NK cell activity,IgG,IgG1,IgG2a and IgG2b antibody titers were all highly significantly elevated and promoted the proliferation and secretion of IL-2 by splenocytes(P<0.01).In conclusion,the adjuvant of flavonoid extracts significantly improved the cellular and humoral immune functions of mice,and the best effect was observed in the middle and high dose groups of PSE50.