多成分定量分析与化学计量学相结合的不同基原厚朴药材辨识

Identification of Different Origins of Magnoliae Officinalis Cortex based on the Combination of Multi-component Quantitative Analysis and Chemometrics

目的:基于多成分定量分析与化学计量学,建立不同基原厚朴药材辨识方法。方法:采用超高效液相色谱法对不同基原厚朴药材中的紫丁香酚苷、木兰花碱、木兰箭毒碱、木兰苷A、木兰苷B、和厚朴酚、厚朴酚和辣薄荷基厚朴酚8个化学成分进行含量测定分析,结合聚类分析、主成分分析、Fisher判别分析、正交偏最小二乘法判别分析,筛选不同基原厚朴药材辨识关键指标,建立厚朴药材基原辨识模型。结果:木兰苷B与木兰苷A含量比值与和厚朴酚与厚朴酚含量比值可作为初步判定基原的指标,而基于Fisher线性判别函数式得分可明显区分不同基原的厚朴药材。结论:基于多成分定量分析与化学计量学相结合可识别不同基原厚朴药材,为多基原药材的辨识提供参考。

Objective:To establish the method for the identification of different origin of Magnoliae Officinalis Cortex based on multi-component quantitative analysis and chemometrics.Methods:Ultra performance liquid chromatography(UPLC)was used to determine the contents of eight chemical components,namely syringin,magnocurarine,magnoflorine,magnoloside B,magnoloside A,honokiol,magnolol and piperitylmagnolol,in different original Magnolia officinalis cortex.Cluster analysis,principal component analysis,Fisher discriminant analysis,and orthogonal partial least squares discriminant analysis were used to screen key identification indicators for different original Magnolia Officinalis Cortex,and a identification model was established.Results:The contents ratios of magnoloside B to magnoloside A and honokiol to magnolol could be used as the key indexes for the preliminary identificaiton of the origin,and the Fisher's Linear Discriminant Functional Score could be clearly distinguished the different origins.Conclusion:The combination of multi-component quantitative analysis and chemometrics can identify different origin of Magnoliae Officinalis Cortex,which can provide a reference for the identification of multi-basal origin herbs.