厨余垃圾废液中解钾菌的筛选及液体菌肥制备

Screening of Potassium Solubilizing Bacteria in Kitchen Waste Liquid and Preparation of Liquid Microbial Fertilizer

从厨余垃圾废液中筛选得到的一株高效解钾菌株LYBM04,解钾效率为65.67%,经16S rDNA确定其为胶质芽孢杆菌(Bacillus mucilaginosus)。LYBM04菌株在LB培养基和解钾菌复筛培养基具有相似的生长曲线,但在解钾菌复筛培养基中整体速度稍慢。通过正交试验,确定了LYBM04菌株可与解磷菌、固氮菌以2∶2∶1(V/V)比例、接种量5%的最优条件下,接入到厨余废液中,30℃好氧发酵40 h制备出液体微生物菌肥,其总磷、氨氮、总氮、总钾等营养成分经过检测,比厨余垃圾废液含量更高,对盆栽玉米的株高、根长、根重均有显著的促生作用,在液态微生物菌肥的开发与应用方面具有一定的应用前景。

This study screened a highly efficient potassium solubilizing bacterial strain LYBM04 from kitchen waste liquid,with a potassium solubilizing efficiency of 65.67%.After 16S rDNA analysis,it was identified as Bacillus mucilaginosus.The LYBM04 strain showed similar growth curves in the LB medium and potassium solubilizing bacteria re-screening medium,but the overall speed was slightly slower in potassium solubilizing bacteria re-screening medium.Through orthogonal experiments,it was determined that the LYBM04 strain can be inoculated into kitchen waste liquid with phosphate solubilizing bacteria and nitrogen fixing bacteria in the optimal ratio of 2∶2∶1 and an inoculation amount of 5%for aerobic fermentation at 30℃for 40 hours to prepare liquid microbial fertilizer.After testing,the total phosphorus,ammonia nitrogen,total nitrogen,total potassium and other nutritional components were detected to be higher than those in kitchen waste liquid.It had a significant promoting effect on the plant height,root length,and root weight of potted corn,and had certain application prospects in the development and application of liquid microbial fertilizer.