夜晚蓝光和白光暴露对肝脏生物钟节律和肝脏炎症反应影响的差异

Differences in the Effects of Night Blue Light and White Light Exposure on Liver Circadian Rhythm and Inflammatory Response

光对昼夜节律的调控属于非成像视觉功能,以自主感光视神经节细胞为主的非成像视觉效应细胞对机体的生物节律、代谢和认知等有显著影响,主要由蓝光介导。该研究探索夜晚蓝光和白光暴露对肝脏生物钟节律以及肝脏炎症反应的影响。将沙鼠每晚暴露在蓝光或白光中1或3 h,持续10周,采用实时荧光定量PCR检测肝脏生物钟基因表达节律,采用转录组学分析肝脏全部基因表达水平,采用H&E染色和免疫荧光法观察肝脏组织形态及炎症因子水平。研究结果显示,夜晚光照对肝脏多种生物钟基因Arntl、Clock、Cry1、Nr1d1、Per2和Ror-α表达节律均造成显著破坏。夜晚光照对肝脏免疫系统基因表达的影响最显著。夜晚光照可引起肝细胞水肿,上调促炎因子白介素17A和粒细胞-巨噬细胞集落刺激因子的表达。夜晚光照暴露扰乱了肝脏生物钟节律并引发肝脏炎症反应。该研究凸显了非成像视觉通路在肝脏代谢稳态中的关键作用。

The regulation of circadian rhythm by light is a non-imaging visual function.Non-imaging visual effect cells,mainly intrinsically photosensitive retinal ganglion cells,significantly impact the body's biological rhythm,metabolism,cognition,etc.,primarilymediated by blue light.This study aimed to explore the effects of night light exposure on liver circadian rhythm and inflammation.The gerbils were exposed to white and blue light for 1 or 3 hours every night for 10 weeks.The real-time reverse transcription-polymerase chain reaction was used to detect the expression rhythm of liver clock genes;transcriptomics was used to analyze the expression level of all liver genes;hematoxylin-eosin staining was used to detect liver tissue morphology,and immunofluorescence staining was used to detect the level of inflammatory factors in liver tissue.The results showed that light exposure at night significantly disrupted the liver's expression rhythm of Arntl,Clock,Cry1,Nr1d1,Per2,and Rora.Light exposure at night had the most significant effect on liver immune system gene expression.Light exposure at night induced hepatocyte edema and up-regulated the expression of pro-inflammatory factors interleukin 17A and granulocyte-macrophage colony-stimulating factor.In short,exposure to light at night disrupted liver circadian rhythms and triggered an inflammatory response in the liver.This study highlights the critical role of non-imaging visual pathways in hepatic metabolic homeostasis.