梭鲈快速生长品系F_(3)基因型与生长性状关联分析

Correlation analysis between genotypes and growth traits of fast growing strain F_(3) of pikeperch(Sander lucioperca)

为了筛选可用于遗传选育的标记,用微卫星标记分析了梭鲈快速生长品系F3群体的遗传结构,评估了不同基因型与生长性状(体质量、全长、体长、体高、体厚、头长、吻长、尾柄高、尾柄长)的相关性。遗传结构评估结果显示:群体平均等位基因数为4.5,观测杂合度为0.746(0.520~0.954),期望杂合度为0.691(0.441~0.851),平均多态信息含量为0.641(0.412~0.843),群体处于高度多态水平(PIC>0.5)。基因型与性状关联分析结果显示38个微卫星标记与至少1个性状表现出不同程度的相关性(P<0.05),其中HLJSL018、HLJSL021、HLJSL022等21个标记与相应性状的相关性达到极显著水平(P<0.01)。9个性状检测到2~21个显著相关的微卫星标记,与体长呈显著相关的标记最少(HLJSL039和HLJSL042)(P<0.05);与尾柄高呈显著相关的标记最多(21个),其中HLJSL018、HLJSL021和HLJSL022等11个标记与其呈极显著相关(P<0.01)。与体质量呈显著相关的标记有8个,其中HLJSL018和HLJSL067与其呈极显著相关(P<0.01)。以高于总体均值且比例达5%以上为优势基因型的筛选标准,在38个标记共获得195个优势基因型,每个标记获得1~10个优势基因型,其中HLJSL073获得优势基因型最少,HLJSL079获得优势基因型最多。本研究评估了梭鲈选育群体的遗传结构,获得了与性状显著相关的标记,研究结果可用于指导梭鲈遗传选育。

In this study,we conducted association analysis between microsatellite markers and growth traits such as body weight,total length,body length,body height,body thickness,head length,snout length,caudal stalk height and caudal length in the F3 population of pikeperch(Sander lucioperca),the fast-growing strain,to identify markers for genetic breeding.The average allele number for each marker was 4.5.The average observed heterozygosity at 0.746(ranging from 0.520 to 0.954),with expected heterozygosity recorded at 0.691(with a range of 0.441 to 0.851).The average polymorphism information content was 0.641.The results showed that 38 microsatellite markers had significant association with at least one trait(P<0.05),among which 21 markers,such as HLJSL018,HLJSL021 and HLJSL022,had extremely significant association with the corresponding trait(P<0.01).Specifically,we detected several 2-21 significant microsatellite markers for 9 traits,ranging from 2 to 21,of which only two markers(HLJSL039 and HLJSL042)were identified for body length(P<0.05);but there are 21 markers significantly associated with the height of the tail stalk,among which 11 markers such as HLJSL018,HLJSL021,and HLJSL022 are highly significant(P<0.01).There are 8 markers significantly associated with body mass,of which HLJSL018 and HLJSL067 are highly significant(P<0.01).A total of 195 dominant genotypes,with a proportion of over 5% higher than the overall mean,were obtained in 38 markers.Each marker obtained 1-10 dominant genotypes,among which HLJSL073 obtained the least dominant genotypes and HLJSL079 obtained the most dominant genotypes.In summary,we assessed the genetic structure of the pikeperch breeding population and identified markers closely associated with growth traits,which can inform the genetic selection of pikeperch.