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RISE Method Based on Rare Allele Infusion and Sanger Sequencing Estimation: A Simple, Cheap, and Efficient Method for Detecting Transgene Copy Number in Rice

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摘要 Detecting exogenous gene copy numbers is crucial for examining genetically modified organisms. Traditional methods face a trade-off between feasibility and cost, highlighting the need for a high-precision and low-cost alternative. In this study, we introduced the RISE method, which is based on Rare Allele Infusion and Sanger Sequencing Estimation, for rice.
出处 《Rice science》 SCIE CSCD 2024年第5期499-502,I0014-I0020,共11页 水稻科学(英文版)
基金 supported by the Natural Science Foundation of Hunan Province for Distinguished Young Scholars, China (Grant No. 2021JJ10041) the Biological Breeding-National Science and Technology Major Project, China (Grant No. 2023ZD04072) the Hainan Yazhou Bay Seed Laboratory and National Seed Group, China (Grant Nos. B23CQ15HP and B23YQ1517)。
关键词 Sanger COST Det
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