Box-Behnken响应面法优选麻贝清肺颗粒提取工艺及其药效学研究

Optimization of Extraction Process of Mabei Qingfei Granules(麻贝清肺颗粒)by Box-Behnken Response Surface Methodology and Study on Its Pharmacodynamics

目的:优化麻贝清肺颗粒提取工艺,评价其治疗急性支气管炎的药理学作用。方法:采用单因素试验筛选加水量、提取时间及浸泡时间对盐酸麻黄碱、新芒果苷、苦杏仁苷、芒果苷、甘草苷、盐酸黄连碱、盐酸小檗碱、盐酸巴马汀、甘草酸含量及干膏率等指标的影响,在此基础上利用Box-Behnken设计(BBD)-响应面法(RSM)结合信息熵理论设计试验并计算综合评分;通过分析回归方程模型,考察影响提取效果的因素,优选麻贝清肺颗粒的提取工艺。采用浓氨水引咳、二甲苯致耳肿胀及气管酚红实验考察麻贝清肺颗粒止咳化痰消炎的药理作用。结果:最佳提取工艺参数为加8.0倍量水,浸泡49.0 min,提取1.8 h。麻贝清肺颗粒可减少浓氨水致咳的次数,降低小鼠耳肿胀,增加酚红分泌量。结论:优化的提取工艺稳定可靠。麻贝清肺颗粒具有明显的止咳、祛痰、抗炎作用。

Objective:To optimize the extraction process of Mabei Qingfei granules,and evaluate the pharmacological effect on the treatment of acute bronchitis.Methods:The effects of water addition,extraction time and soaking time on the contents of ephedrine hydrochloride,nemangiferin,amygdalin,mangiferin,liquiritin,coptisine hydrochloride,berberine hydrochloride,palmatine hydrochloride,glycyrrhizic acid and dry paste ratio were screened by single factor test.On this basis,the Box-Behnken response surface method combined with information entropy theory was used to design the experiment and calculate the comprehensive score.According to analyzing the regression equation model,the factors affecting the extraction effect were investigated,and the extraction process of Mabei Qingfei granules was optimized.The pharmacological effects of Mapei Qingfei granules on cough,phlegm and inflammation were studied by using concentrated ammonia water to induce cough,ear swelling caused by xylene and tracheal phenol red.Results:The optimum extraction parameters were added 8.0 times water,soaked for 49.0 min and extracted for 1.8 h.Mabei Qingfei granules can significantly reduce the number of cough caused by concentrated ammonia water,reduce ear swelling and significantly increase the secretion of phenol red in mice.Conclusion:The optimized extraction process is stable and reliable.Mabei Qingfei granules shows obvious antitussive,antiphlegm and anti-inflammatory effects.