满山红质量标志物含量测定及最佳采收期研究

Determination of Content of Quality Markers of Rhododendron Dauricum L. and Study on Its Optimal Harvesting Period

目的:建立满山红药材质量标志物含量测定方法,依此对不同采收时期质量进行评价。方法:采用Agilent Extend-C18色谱柱,流动相为0.2%磷酸(A)-乙腈溶液(B)梯度洗脱,流速为1.0 mL/min,柱温为35℃,检测波长为360 nm(0~35 min)和296 nm(35~45 min),进样量为10μL。结果:金丝桃苷、槲皮素、山柰酚、8-去甲杜鹃素、杜鹃素分别在12.32~123.20、20.00~200.00、3.28~32.80、9.32~93.20、120.56~1 205.60μg/mL与峰面积线性关系良好,平均加样回收率分别为99.70%~103.96%(RSD=1.85%)、99.13%~102.61%(RSD=1.71%)、98.10%~102.86%(RSD=1.76%)、98.29%~100.85%(RSD=0.90%)、98.76%~100.62%(RSD=0.66%);确定7月份为最佳采收期。结论:采用高效液相色谱(HPLC)法同时建立了金丝桃苷、槲皮素、山柰酚、8-去甲杜鹃素、杜鹃素的测定方法,使满山红的质量评价方法更便捷、更全面,以此评价的最佳采收时期方法合理,可为保证满山红药材质量及促进其开发利用提供实验依据。

Objective:To establish a method for determining the content of quality marker based on network pharmacology research for Rhododendron dauricum L.,and evaluate different harvesting periods accordingly.Methods:Agilent Extend-C18 chromatographic column was used,with a flow phase of 0.2%phosphoric acid(A)-acetonitrile solution(B)for gradient elution.The flow rate was 1.0 mL/min,with column temperature of 35℃,and the detection wavelength was 360 nm(0-35 min)and 296 nm(35-60 min).The injection amount was 10μL.Results:There was good linear relationship between peak area and the concentrations of Hyperoside,Quercetin,Kaempferol,8-norrhododendron and rhododendron respectively at 12.32-123.20,20.00-200.00,3.28-32.80,9.32-93.20,120.56-1205.60μg/mL.The average recovery rates were respectively from 99.70-103.96%(RSD=1.85%),99.13%-102.61%(RSD=1.71%),98.10%-102.86%(RSD=1.76%),98.29%-100.85%(RSD=0.90%)and 98.76%-100.62%(RSD=0.66%).July is the optimal harvesting period.Conclusions:A HPLC method for the determination of hyperoside,Quercetin,Kaempferol,8-demethylrhododendron and rhododendron was established simultaneously,which makes the quality evaluation of Rhododendron dauricum L.more convenient and comprehensive.The best harvest time evaluated by this method is reasonable,providing experimental basis for the secondary processing and application of Rhododendron.