实时荧光定量PCR仪用于流感病毒检测的效果分析

Analysis of the Effectiveness of Real-Time Fluorescence Quantitative PCR Instrument for Influenza Virus Detection

目的:探讨流感病毒检测中应用实时荧光定量PCR仪的价值。方法:选取2022年1月~2023年12月本中心采集检测的129份急性上呼吸道感染者的咽拭子标本展开研究,采集标本后实施实时荧光PCR仪、胶体金法测定标本中流感病毒核酸,分析两种方案检出情况、诊断效能。结果:实时荧光定量PCR仪阳性率19.38%,胶体金法阳性率为14.73%,实时荧光定量PCR仪检测阳性率高于胶体金法(P<0.05)。实时荧光定量PCR仪特异度(99.02%)、灵敏度(92.59%)、准确率(97.67%)高于胶体金法(P<0.05)。结论:在流感病毒检测中应用实时荧光定量PCR仪准确率、敏感度较高,且该方案可以迅速且有效检测出流感病毒核酸,为迅速地采取有效的疫情防控措施提供依据。

Objective:To explore the value of real-time fluorescent quantitative PCR(FQ-PCR)in detection of influenza virus.Methods:129 throat swab samples of acute upper respiratory tract infections collected and tested by our center from January 2022 to December 2023 were selected for the study.After collecting the samples,real-time fluorescence PCR and colloidal gold methods were used to measure influenza virus nucleic acid in the samples,and the detection and diagnostic efficacy of the two schemes were analyzed.Results:The positive rate of real-time fluorescent quantitative PCR was 19.38%,and that of colloidal gold method was 14.73%.The positive rate of real-time fluorescent quantitative PCR was higher than that of colloidal gold method(P<0.05).The specificity(99.02%),sensitivity(92.59%)and accuracy(97.67%)of real-time fluorescent quantitative PCR were higher than those of colloidal gold method(P<0.05).Conclusion:The accuracy and sensitivity of real-time fluorescent quantitative PCR in detection of influenza virus are high,and the method can detect influenza virus nucleic acid quickly and effectively,to provide the basis for taking effective epidemic prevention and control measures.