嗜黏蛋白阿克曼氏菌ONE对高胆固醇血症小鼠的改善效果

Alleviating Effect of Akkermansia muciniphila ONE on Hypercholesterolemia in Mice

研究嗜黏蛋白阿克曼氏菌ONE(Akkermansia muciniphila ONE,AKK ONE)对高胆固醇饮食小鼠的改善作用。对高胆固醇饮食小鼠灌胃AKK ONE 4周后发现,AKK ONE能够改善高胆固醇血症小鼠的血脂水平,显著降低高胆固醇血症小鼠血清和肝脏总胆固醇、低密度脂蛋白胆固醇含量,升高高密度脂蛋白胆固醇含量,改善肝脏损伤,降低血清谷丙转氨酶、谷草转氨酶和总胆酸水平,改善空腹血糖水平和葡萄糖耐量。进一步肠道菌群和代谢物分析显示,AKK ONE显著增加了小鼠肠道内Faecalibaculum、Oscillospira、Bacteroides、Akkermansia的丰度,增加了短链脂肪酸(short-chain fatty acids,SCFAs)包括乙酸、异丁酸、戊酸的含量,调节了肠道内胆汁酸(bile acids,BAs)尤其是次级BAs的组成。总之,AKK ONE通过调节肠道菌群丰度,尤其增加是产SCFAs的菌群,进而增加SCFAs含量,增强胆固醇向BAs的转化,进而改善高胆固醇饮食所引起的高胆固醇血症。本研究探讨了下一代益生菌与肠道菌群、代谢物之间的相互关系,为AKK ONE改善高胆固醇血症方面的潜在应用提供了参考依据。

The purpose of this study was to investigate the alleviating effect of Akkermansia muciniphila ONE(AKK ONE)on high-cholesterol diet-induced hypercholesterolemia in mice.Four-week gavage of AKK ONE was found to improve blood lipid levels in hypercholesterolemic mice.Specifically,it significantly reduced the contents of total cholesterol(TC)and low-density lipoprotein cholesterol(LDL-C)in serum and liver,increased the content of high-density lipoprotein cholesterol(HDL-C),alleviated liver damage,reduced the levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)and total bile acid(TBA),and improved fasting blood glucose levels and glucose tolerance.Further analysis of intestinal flora and metabolites showed that AKK ONE significantly increased the abundance of Faecalibaculum,Oscillospira,Bacteroides and Akkermansia in the intestine of mice,increased the contents of short-chain fatty acids(SCFAs)including acetic acid,isobutyric acid and valeric acid,and regulated the composition of intestinal bile acids(BAs)especially secondary BAs.In summary,AKK ONE improved high cholesterol diet-induced hypercholesterolemia by regulating the abundance of intestinal flora,especially increasing the abundance of SCFAs-producing bacteria,thereby increasing the contents of fecal SCFAs and enhancing the transformation of cholesterol into BAs.This study provides a new insight into the relationship between next-generation probiotics and intestinal flora and metabolites,which will serve as the basis for the potential application of AKK ONE in alleviating hypercholesterolemia.