生菜HSF基因家族的全基因组鉴定与表达分析

Genome-wide identification and expression analysis of HSF gene family in lettuce

利用生菜的全基因组数据,采用生物信息学方法,从生菜中鉴定出了35个LsHSF基因,按照其在染色体上的分布顺序命名为LsHSF1,LsHSF2,…,LsHSF35,并对其理化性质、染色体定位、与拟南芥的进化关系、蛋白保守基序、组织表达水平及褪黑素处理后高温条件下的表达量进行分析。亚细胞定位预测分析结果显示,除LsHSF11、LsHSF17定位在叶绿体,LsHSF34定位在液泡外,其余基因都定位在细胞核中;35个LsHSF基因不均等地分布在9条染色体上;蛋白保守基序预测分析结果显示,所有LsHSF基因都含有Motif 1与Motif 2,且存在紧密联系;系统发育树分析结果表明,HSF蛋白分为A、B和C共3组,被划分为14个主要亚家族;组织表达水平分析结果表明,HSF等基因具有特异性表达模式;高温处理能够刺激HSF基因家族成员的表达。

Using the whole-genome data of lettuce,35 LsHSFgenes were identified from lettuce through bioinformatics methods,and named LsHSF1,LsHSF2,…,LsHSF35according to thedistribution order on theirchromosome.Their physicochemical properties,chromosomal localization,evolutionary relationship with Arabidopsis,protein conserved motifs,tissue expression levels,and expression levels under high-temperature conditions after melatonin treatment were further analyzedin this study.The results showed that subcellular localization prediction analysis indicated that,except for LsHSF11and LsHSF17located in chloroplasts,and LsHSF34 located outside the vacuole,the rest of the genes were located in the nucleus.The 35LsHSF genes were unevenly distributed on 9 chromosomes.Protein conserved motif prediction analysis revealed that all LsHSF genes containedtwo closely relatedMotif 1 and Motif 2.From phylogeneticanalysis,HSF proteins were divided into threegroups:A,B,and Cand could befurther subdivided into 14 major subfamilies.Tissue expression level analysis results indicated that HSFgenes exhibited specific expression patterns.High-temperature treatment could stimulate the expression of HSF gene family members.