苦荞FtDELLA基因的克隆与表达分析

Cloning and expression analysis of Fagopyrum tataricum FtDELLA gene

DELLA蛋白是赤霉素(GA)传导途径中的负调控蛋白,其在植物生长发育和胁迫响应中起着重要作用。该实验利用RT-PCR技术从苦荞中克隆到一个DELLA基因,命名为FtDELLA。克隆得到FtDELLA基因CDS全长1 452 bp,编码483个氨基酸。蛋白多序列比对显示,FtDELLA蛋白含有DELLA蛋白家族保守的DELLA保守结构域;系统进化分析表明,FtDELLA蛋白与拟南芥中AtRGA1亲缘关系最近。qRT-PCR结果显示,FtDELLA在苦荞不同组织部位均有表达,其中在茎和灌浆前的种子中表达量最高,在苦荞种子发育过程中,FtDELLA表达量呈逐渐下降趋势。在干旱胁迫下,FtDELLA的表达明显受到抑制,表明其可能是苦荞干旱胁迫应答的一个负调控基因。该研究结果为进一步研究FtDELLA在苦荞中的功能和作用机制提供了理论基础。

DELLA protein is a negative regulatory protein in the gibberellin(GA)transduction pathway,which plays an important role in plant growth and development and stress response.In this experiment,a DELLA gene,named FtDELLA,was cloned from buckwheat using RT-PCR.The gene is 1452 bp in CDS length and encodes 483 amino acids.Phylogenetic analysis showed that the FtDELLA protein contained the conserved DELLA conserved structural domains of the DELLA family.The qRT-PCR results showed that FtDELLA was highly expressed in stems and pre-grouting seeds,and its expression showed a decreasing trend during seed development.Under drought stress,the expression of FtDELLA showed a decreasing trend,indicating that it may be a negative regulatory gene for drought stress response in buckwheat.The results suggest that FtDELLA may play an important regulatory role in the growth and development of buckwheat and drought stress response.The results provide a theoretical basis for further research on the function and mechanism of action of FtDELLA in buckwheat.