不同地区枸杞真菌毒素含量特征分析

Analysis of mycotoxin content characteristics of Lycium barbarum L.in different areas

目的建立QuEChERS-超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)检测新疆、甘肃、宁夏3个地区枸杞中链格孢酚(alternariol,AOH)、腾毒素(tentoxin,TEN)、交链孢霉烯(altenuene,ALT)3种真菌毒素含量,并对3个地区真菌毒素含量进行特征分析。方法本研究采用改良的QuEChERS-UPLC-MS/MS对枸杞中真菌毒素进行测定,分析新疆、甘肃、宁夏3个地区枸杞中真菌毒素分布特征。结果90份枸杞样品中AOH检出率为55.6%,TEN检出率为100%,ALT检出率为100%,79.7%的枸杞样品受到了两种以上真菌毒素污染,在新疆、甘肃、宁夏3地区真菌毒素含量中ALT总含量(48.3%)>AOH总含量(47.3%)>TEN总含量(4.3%)。结论该方法简单准确,适用于枸杞中真菌毒素的同时检测;ALT、TEN的检出率较高;混合污染情况普遍,并且呈现出一定的地区和品种差异。通过分析不同地区枸杞中真菌毒素的含量特征,可以及时发现并控制真菌毒素污染,保障消费者食用安全。同时也可以预测和预防可能发生的食品安全事故,为监管部门提供科学依据。

Objective To establish a QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was ed for the determination of alternariol(AOH),tentoxin(TEN)and altenuene(ALT)in Lycium barbarum L.from Xinjiang,Gansu and Ningxia.The characteristics of mycotoxin content in three regions were analyzed.Methods In this study,the modified QuEChERS-UPLC-MS/MS was used to determine the mycotoxins in Lycium barbarum L.,and the distribution characteristics of mycotoxins in Lycium barbarum L.in Xinjiang,Gansu and Ningxia were analyzed.Results The detection rate of AOH was 55.6%,the detection rate of TEN was 100%,and the detection rate of ALT was 100%.The 79.7%of the samples were polluted by more than 2 kinds of mycotoxins.In Xinjiang,Gansu and Ningxia,the total content of ALT(48.3%)>the total content of AOH(47.3%)>the total content of TEN(4.3%).Conclusions The method is simple and accurate,and is suitable for the simultaneous detection of mycotoxins in Lycium barbarum L..The detection rates of ALT and TEN are high.Mixed pollution is common and presents certain regional and variety differences.By analyzing the content characteristics of mycotoxins in Lycium barbarum L.in different regions,mycotoxin contamination can be detected and controlled in time to ensure consumer safety.At the same time,it can also predict and prevent possible food safety accidents and provide scientific basis for regulatory authorities.