聚丙烯酸-钙(铈)纳米团簇荧光探针用于无机磷定量检测研究

Polyacrylate-calcium(cerium)Nanocluster Fluorescent Probes for Quantitative Detection of Inorganic Phosphorus

无机磷主要以磷酸根的形式存在,在自然界生命循环和现代工业社会中发挥重要作用。磷酸根离子的快速高效定量检测一直是临床生化分析、工业生产和环境污染监测等领域的研究热点。本工作以聚丙烯酸(PAA)为络合剂,通过与Ca^(2+)和Ce^(3+)的络合反应,合成了具有良好分散性和稳定性的PAA-Ca(Ce)纳米团簇荧光探针。利用298 nm激发光照射该探针与磷酸根的反应产物,建立其在352nm处发射峰峰值强度与磷酸根浓度的线性关系图。实验结果表明,(Ca^(2+)+Ce^(3+))浓度为37.575 mmol/L的纳米荧光探针与磷酸根浓度的线性关系式为y=1.09x+2.05,荧光强度可靠性范围为13.5~66.91 mmol/L。与钼锑抗分光光度法测试磷酸根对比,该方法具有更高的回收率。大鼠血清无机磷检测实验验证了该方法的可靠性。以上结果表明本研究所合成的荧光探针具有良好的磷酸根定量检测性能。

Phosphorus is one of the important elements in the ecosystem and plays a vital role in the process of life cycle.Inorganic phosphorus is a major form of phosphorus,usually in the form of phosphate ions.The rapid and efficient quantitative detection of phosphate ions has always been a hot research direction in the fields of clinical chemistry,pharmacology,biochemical analysis,industrial production and environmental pollution monitoring,but still facing some challenges in accuracy and convenient in some special circumstances.In present work,PAA-Ca(Ce)nanocluster fluorescent probes with good dispersibility and stability were synthesized by complexing with Ca^(2+)and Ce^(3+)using polyacrylic acid(PAA)as complexing agent.The reaction product between the probe and the phosphate ion was irradiated by 298 nm excitation light,and a linear graph was established based on the correlation between the peak intensity at the emission peak of 352 nm and the phosphate ion concentration.The experimental results show that the linear relation of the nanofluorescent probe with the(Ca^(2+)+Ce^(3+))concentration of 37.575 mmol/L is y=1.09x+2.05,and the reliability range of fluorescence intensity is 13.5-66.91 mmol/L.Compared with molybdenum-antimony resistance spectrophotometry,this method has a higher recovery.The experimental results of inorganic phosphorus in rat serum verify the reliability of the method.The results above indicate that the fluorescence probe studied in this paper has good quantitative detection performance of phosphate.