链脲佐菌素联合高糖高脂饮食建立糖尿病胃轻瘫大鼠模型及其评价

Establishment of diabetic gastroparesis rat model by streptozotocin combined with high sugar and high fat diet and its evaluation

目的探讨建立糖尿病胃轻瘫(diabetic gastroparesis,DGP)动物模型的方法。方法从25只正常SD大鼠中随机选择15只进行造模,造模大鼠腹腔单次注射50 mg/kg的2%链脲佐菌素(streptozotocin,STZ)溶液建立糖尿病模型。糖尿病大鼠造模成功后给予高糖高脂饲料不规则喂养6周,设为模型组。10只大鼠作为对照组,给予腹腔单次注射0.1 mol/L柠檬酸钠缓冲液(pH=4.5)并给予基础饲料喂养6周。观察大鼠一般状况、体质量变化、随机血糖变化、胃肠动力指标以及HE染色胃窦部形态学变化。结果造模后,与对照组相比,模型组大鼠体质量明显降低(P<0.01),随机血糖显著升高(P<0.01),胃排空率降低(P<0.01),小肠推进率降低(P<0.05);胃窦部组织腺体排列杂乱,出现炎性细胞浸润。结论腹腔单次注射2%的50 mg/kg STZ联合高糖高脂饲料不规则喂养6周可建立DGP大鼠模型。

Objective To explore the method of establishing the animal model of diabetic gastroparesis(DGP).Methods Fifteen out of 25 normal SD rats were randomly selected for modeling.The rats were injected with 50 mg/kg 2%streptozotocin(STZ)solution once intraperitoneally to establish the diabetes model.After successful modeling,diabetic rats were fed with high-sugar and high-fat diet for 6 weeks and were set as model group.Ten rats as control group were given a single intraperitoneal injection of 0.1 mol/L sodium citrate buffer(pH=4.5)and fed basic diet for 6 weeks.The general condition,body weight changes,random blood glucose changes,gastrointestinal dynamics indexes and morphological changes of HE-stained gastrointestinal sinus section were observed in the two groups of rats.Results After modeling,compared with the control group,rats in the model group had significantly lower body weight(P<0.01),significantly higher random blood glucose(P<0.01),lower gastric emptying rate(P<0.01)and lower propulsion rate of the small intestine(P<0.05);and disorganized arrangement of the tissue glands in the gastric sinus department,with inflammatory cell infiltration.Conclusion DGP rats model can be established by a single intraperitoneal injection of 2%50 mg/kg STZ combined with high-sugar and high-fat diet for 6 weeks.