基于高通量测序探究双歧杆菌四联活菌片对非酒精性脂肪性肝病患者肠道菌群失衡的干预作用

Effects of Bifidobacterium tetragenous viable bacteria tablets on gut microbiota in non-alcoholic fatty liver disease patients based on high-throughput sequencing

目的探讨双歧杆菌四联活菌片对非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)患者肠道菌群的影响。方法纳入2021年7月至2022年1月在我院收治的患有NAFLD的患者20例作为NAFLD组,同期来院健康检查的受试者20名作为健康对照组。NAFLD组给予口服双歧杆菌四联活菌片4周,在治疗前后检测肝功能及血脂水平,并进行粪便样本采集。对NAFLD患者治疗前后及健康对照的粪便样本进行16S rDNA测序,分析肠道菌群的组成和结构。结果在双歧杆菌治疗后,NAFLD患者的肝功能指标ALT、AST及ALP,血脂指标TC及HDL显著改善。双歧杆菌未改变NAFLD组患者菌群的Alpha多样性,但Beta多样性发生显著改变。在双歧杆菌治疗后,NAFLD患者肠道中放线菌显著增加,而厚壁菌显著降低。Bifidobacterium、Bacteroides显著增加,Eubacterium_coprostanoligenes显著降低。LEfSe分析识别出双歧杆菌治疗后的生物标志物Bifidobacteriaceae、Actinobacteria、Coriobacteriaceae及Enterobacteriaceae。结论双歧杆菌四联活菌片可以调节NAFLD患者的肝功能及血脂代谢并改善肠道菌群失调,具有一定的临床应用前景。

Objective To investigate the effect of Bifidobacterium tetragenous viable bacteria tablets on gut microbiota in non-alcoholic fatty liver disease(NAFLD)patients.Methods Twenty patients with NAFLD admitted to our hospital from Jul.2021 to Jan.2022 were enrolled as the NAFLD group,and 20 subjects who came to our hospital for health examination during the same period were enrolled as healthy control group.NAFLD patients were given bifidobacterium tetragenous viable bacteria tablets orally for 4 weeks.Liver function and blood lipid levels were detected,and fecal samples were collected before and after treatment.Fecal samples of NAFLD patients and healthy controls were detected by 16S rDNA sequencing to analyze the composition and structure of gut microbiota.Results Bifidobacterium treatment significantly improved the levels of ALT,AST,ALP,TC and HDL in NAFLD patients.Bifidobacterium treatment did not alter the Alpha diversity of NAFLD patients,but the Beta diversity was significantly altered.After Bifidobacterium treatment,there was a significant increase in Actinobacteria and a significant decrease in Firmicutes of NAFLD patients.Bifidobacterium and Bacteroides were significantly increased,and Eubacterium_coprostanoligenes were significantly decreased.LEfSe analysis identified biomarkers of Bifidobacteriaceae,Actinobacteria,Coriobacteriaceae,and Enterobacteriaceae after Bifidobacteriaceae treatment.Conclusion Bifidobacterium tetragenous viable bacteria tablets can regulate liver function and lipid metabolism and improve gut microbiota imbalance in NAFLD patients,which has a certain clinical application prospect.