杀菌剂复配对羊肚菌细菌性病原菌假单胞菌的室内活性测定及田间防效

Laboratory activity and field control efficacy of fungicide combination against Pseudomonas,a bacterial pathogen of Morchella

[目的]研究针对羊肚菌Morchella spp.细菌性病原菌假单胞菌Pseudomonas的高效防治策略。[方法]自贵州本土染病羊肚菌子实体中分离纯化出致病菌假单胞菌。采用浊度法研究14种商品农药及不同复配组合对假单胞菌的室内生物活性,并通过大田试验进一步验证其防效。[结果]室内生物活性测定结果显示将20%辛菌胺醋酸盐AS和80%乙蒜素EC以3∶1的比例复配具有增效作用,SR为2.0229,其他组合及配比均为相加作用或拮抗作用。大田试验结果表明将20%辛菌胺醋酸盐AS与80%乙蒜素EC以3∶1进行复配具有较好的防效,在质量浓度为12 mg/L时,防治效果达到78.64%±2.05%。[结论]将20%辛菌胺醋酸盐AS和80%乙蒜素EC以3∶1的比例复配对于因假单孢菌引起的羊肚菌细菌性病害具有较好的防治效果。

[Aims]The aim is to study on the effective control strategies against Pseudomonas,a bacterial pathogens of Morchella spp.[Methods]The pathogenic bacterium Pseudomonas was isolated and purified from the fruit bodies of infected Morchella in Guizhou Province.The turbidity method was used to study the laboratory bioactivity against Pseudomonas of 14 commercial pesticides and their different combinations,and the control efficacy was further verified by field trial.[Results]The results of laboratory bioactivity test showed that the combination of seboctylamine acetate 20%AS and ethylicin 80%EC in a ratio of 3∶1 had a synergistic effect,with the SR of 2.0229.Other combinations and ratios had additive or antagonistic effects.The results of field trial showed that the combination of seboctylamine acetate 20%AS and ethylicin 80%EC in a ratio of 3∶1 had a good control efficacy,with a control effect reaching 78.64%±2.05%when the concentration was 12 mg/L.[Conclusions]The combination of seboctylamine acetate 20%AS and ethylicin 80%EC in a ratio of 3∶1 has a good control effect on bacterial disease of Morchella spp.caused by Pseudomonas.