干旱胁迫下土壤微生物对白羊草生长及生理生化特征的影响

Effects of Soil Microbes on the Growth Physiological and Biochemical Characteristics of Bothriochloa ischaemum under Drought Stress

以‘太行’白羊草(Bothriochloa ischaemum)为材料,通过盆栽试验探究了土壤微生物对干旱胁迫下白羊草幼苗植株生长及生理生化的影响。结果表明:与未灭菌土壤处理相比,干旱和非干旱条件下土壤灭菌处理显著降低了植株的株高、茎粗、地上部和地下部干鲜重及叶绿素含量,提高了超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化物酶(Peroxidase,POD)、过氧化氢酶(Catalase,CAT)活性以及丙二醛(Malondialdehyde,MDA)、可溶性蛋白和可溶性糖含量。然而在持续干旱条件下,添加土壤菌悬液处理的白羊草幼苗植株的株高、地上部鲜干重和地下部干重、总叶绿素含量、可溶性糖和可溶性蛋白含量显著提高;SOD,POD活性和MDA含量显著降低。说明土壤微生物能够有效缓解干旱胁迫对白羊草幼苗生长的抑制作用,本研究对进一步认清土壤微生物对白羊草生长和抗旱作用的影响以及对微生物菌种资源的开发利用具有重要意义。

Using‘Taihang’Bothriochloa ischaemum as the materiala,a pot experiment was conducted to explore the effects of soil microbes on the growth,physiological and biochemical characteristics of Bothriochloa ischaemum seedlings under drought stress.The results showed that compared with the unsterilized soil treatment,soil sterilization significantly reduced plant height,stem diameter,dry and fresh weight(aboveground and underground),and chlorophyll content,and increased the activity of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),malondialdehyde(MDA),soluble protein and soluble sugar content under arid and non-arid conditions.Under drought stress,the addition of soil microbial suspension significantly increased the plant height,aboveground fresh and dry weight,underground dry weight,total chlorophyll content,soluble sugar and soluble protein contents of the seedlings.It also significantly reduced the activity of SOD,POD and MDA content.This indicated that soil microbes could effectively alleviate the inhibition of drought stress on the growth of Bothriochloa ischaemum seedlings.This study is of great significance for further understanding the effects of soil microbes on the growth and drought resistance of Bothriochloa ischaemum,as well as for the development and utilization of microbial resources.