原发性肝癌血清miR-375、miR-25水平变化及其与患者预后关系的研究

Changes of Serum miR-375 and miR-25 Levels and Their Relationship with Prognosis of Patients with Primary Liver Cancer

目的 探究原发性肝癌(HCC)患者血清miR-375、miR-25水平变化情况及其i与预后的关系。方法 选取2020年3月至2021年3月本院收治的50例HCC患者为观察组,另选取同期30例健康体检者为对照组,采用RT-qPCR检测血清miR-375、miR-25水平,探究HCC患者治疗前后miR-375、miR-25水平变化及其在HC患者预后.诊断中的价值,并分析HCC患者1年生存期的独立影响因素。结果 治疗前,观察组HCC患者miR-375水平低于对照组,其miR-25水平高于对照组(P<0.05);治疗1个月后,观察组HCC患者miR-375水平高于治疗前,其miR-25水平低于治疗前(P<0.05),且其miR-375低于对照组,miR-25高于对照组(P<0.05)。50例HCC患者均接受TACE治疗,其1年生存率为82.00%(41/50)。miR-375诊断HCC患者1年生存期的AUC、灵敏度和特异度分别为0.736(0.626-0.828)、84.00%和63.33%(P<0.05);miR-25诊断HCC的AUC、灵敏度和特异度分别为0.782(0.676-0.867)、80.00%和66.67%(P<0.05)。病灶大小、miR-375、miR-25表达量是HCC患者预后的独立影响因素(P<0.05)。结论 miR-375、miR-25在HCC患者中表达异常,在治疗后miR-375升高,miR-25降低,病灶小、miR-375、miR-25表达量是HCC患者预后的影响因素。

Objective To investigate the changes of serum miR-375 and miR-25 levels in patients with primary liver cancer(HCC)and their relationship with prognosis.Methods 50 HCC patients admitted to our hospital from March 2020 to March 2021 were selected as the observation group,and 30 healthy subjects during the same period were selected as the control group.Serum miR-375 and miR-25 levels were detected by RT-qPCR.To explore the changes of miR-375 and miR-25 levels in HCC patients before and after treatment and their value in the prognosis of HC patients,and analyze the independent influencing factors on the 1-year survival of HCC patients.Results Before treatment,the miR-375 level of HCC patients in observation group was lower than that in control group,and the miR-25 level was higher than that in control group(P<0.05).After 1 month of treatment,the miR-375 level of HCC patients in the observation group was higher than before treatment,and the miR-25 level was lower than before treatment(P<0.05),and the miR-375 level was lower than that of the control group,and the miR-25 level was higher than that of the control group(P<0.05).All 50 HCC patients received TACE treatment,and the 1-year survival rate was 82.00%(41/50).The 1-year survival AUC,sensitivity and specificity of miR-375 in HCC patients were 0.736(0.626-0.828),84.00%and 63.33%(P<0.05).The AUC,sensitivity and specificity of miR-25 in the diagnosis of HCC were 0.782(0.676-0.867),80.00%and 66.67%(P<0.05).Lesion size,miR-375 and miR-25 expression levels were independent prognostic factors of HCC patients(P<0.05).Conclusion The expression of miR-375 and miR-25 is abnormal in HCC patients,and miR-375 is increased and miR-25 is decreased after treatment.Lesion size,miR-375 and miR-25 expression level are prognostic factors of HCC patients.