淹水胁迫下葡萄根际细菌群落结构变化

Changes in structure of rhizobacteria community in‘Kyoho’grapevine under flooding stress

为探讨根际细菌和葡萄耐涝性的关系,采用盆栽淹水法,对1年生巨峰葡萄扦插苗进行淹水胁迫21d以及淹水后恢复7 d的处理,运用16S rRNA高通量测序技术对土壤细菌进行测序,分析其细菌群落组成和结构,探究淹水胁迫对巨峰葡萄根际细菌群落结构组成的影响。结果显示:淹水21 d巨峰葡萄根际细菌的丰度最高,淹水14 d巨峰葡萄的根际细菌多样性最高;胁迫后恢复期间,硫杆菌属(Thiobacillus)相对丰度显著增加;根系细菌功能预测分析显示脂质、萜类和聚酮类化合物代谢有显著变化。结果表明,短期淹水胁迫促进植物产生代谢物改变细菌群落以应对逆境胁迫,但长时间淹水使植物代谢发生紊乱,导致潜在有害细菌类群的丰度增加,植物有益菌群减少。

The pot flooding method was used to study the waterlogging tolerance of grapevine to mine and utilize the plant rhizobacteria and their functions.Cuttings of 1-year-old Kyoho grapevine were subjected to flooding stress for 21 days and recovery for 7 days after flooding.16S rRNA high-throughput sequencing technol⁃ogy was used to sequence bacteria in soil,analyze the composition and structure of bacterial community,and study the effects of flooding stress on the composition of rhizobacteria community in Kyoho grapevine.The re⁃sults showed that the abundance of rhizobacteria in Kyoho grapevine was the highest after 21 days of flooding,and the diversity of rhizobacteria in Kyoho grapevine was the highest after 14 days of flooding.The relative abun⁃dance of Thiobacillus increased significantly during the recovery period after stress.The results of functional pre⁃diction of root bacteria showed that there were significant changes in the metabolism of lipid,terpenoid and polyketide.It is indicated that short-term flooding stress promotes the production of metabolites in plants to change bacterial communities in response to stress,but prolonged flooding disrupts plant metabolism,leading to an increase in the abundance of potentially harmful bacterial flora and a decrease in beneficial bacterial flora in plants.It will provide theoretical basis for the mining and utilization of rhizobacteria and their functions.