阴道卷曲乳杆菌S层蛋白多样性研究

Diversity of S-layer proteins in vaginal lactobacillus crispatus

目的:分析比较西安市女性阴道卷曲乳杆菌S层蛋白的多样性及NCBI数据库中阴道来源卷曲乳杆菌S层蛋白的多样性。方法:选取100例妇科患者的阴道分泌物标本作为研究对象,从其中10例标本中分离得到480株卷曲乳杆菌,其中两株卷曲乳杆菌S层蛋白有明显差异,对其进行全基因组测序;并从NCBI数据库中下载人阴道源的卷曲乳杆菌全基因组数据,分析并比较西安市来源与数据库来源的卷曲乳杆菌S层蛋白的多样性。结果:S层蛋白注释基因具有菌株内和菌株间的多样性,同一套基因组中有多个S层蛋白注释基因,菌株间的相似性偏差较大,通过系统发育分析发现,中国人阴道卷曲乳杆菌的S层蛋白基因与其他人种的具有系统发育相似性。结论:阴道卷曲乳杆菌S层蛋白具有菌种间和菌株间多样性的特征,不同人种的卷曲乳杆菌具有相似性。

Objective:To analyze and compare the diversity of S-layer proteins of Lactobacillus crispatus from women's vaginas in Xi an and the diversity of S-layer proteins of L.crispatus from vaginal sources in the NCBI database.Methods:100 specimens of vaginal secretions from patients seen in obstetrics and gynecology were selected,480 strains of curly lactobacilli were isolated from 10 specimens,among which two strains showed significant differences in the S layer protein.Whole genome sequencing was performed on them.And download the whole genome data of human vaginal derived Lactobacillus crispus from NCBI database,the diversity of S-layer proteins of Lactobacillus.Results:The S-layer protein annotation genes had intra-and inter-strain diversity,with multiple S-layer protein annotation genes in the same set of genomes and large similarity bias between strains.Phylogenetic analysis revealed that the S-layer protein genes of Lactobacillus crispus vaginalis of Chinese origin had phylogenetic similarity with those of other human species.Conclusion:The S-layer proteins of Lactobacillus crispus are characterized by interspecies and interstrain diversity,and Lactobacillus crispus from different human species have similarities.