摘要
为探究非生物胁迫对结球甘蓝生长的影响,以甘蓝“京丰一号”为材料,利用脱落酸(ABA)、聚乙二醇(PEG)、氯化钠(NaCl)对结球甘蓝幼苗进行处理,分析不同处理对幼苗叶绿素的质量分数,过氧化物歧化酶(SOD)、过氧化物酶(POD)活性,丙二醛(MDA)浓度的影响.结果表明,在各胁迫初期,甘蓝幼苗叶片叶绿素的质量分数均增加,并在ABA,PEG胁迫的第6 h或NaCl胁迫的第12h达到峰值,而后下降.在PEG和NaCl胁迫处理6h时,ABA处理的第12 h,SOD,POD活性均达到最高值,处理时间继续延长,酶活性开始下降.而MDA浓度随着各种处理时间的增加逐渐升高,说明胁迫时间越长植物受损越严重.研究显示,甘蓝可以通过激活抗氧化系统以抵抗胁迫初期活性氧的积累,当植物无法抵抗胁迫时,活性氧动态失去平衡导致生物膜受损.
In order to explore the effect of abiotic stress on the growth of Brussels sprouts,used Cabbage“Jingfeng No.1”as the material to treat Cabbage seedlings with abscisic acid(ABA),polyethylene glycol(PEG)and sodium chloride(NaCl),and analyzed the chlorophyll quality score of the seedlings under different treatments.Peroxide dismutase(SOD),peroxidase(POD)activity,effect of malondialdehyde(MDA)concentration.The results showed that the chlorophyll quality score in the leaves of cabbage seedlings increased at the early stage of each stress,and reached a peak on the 6th h of ABA and PEG stress or the 12th h of NaCl stress,and then decreased.The activities of SOD and POD reached the highest values at 6 h of PEG and NaCl stress treatment and 12 h of ABA treatment,and the treatment time continued to prolong,and the enzyme activities began to decrease.The concentration of MDA gradually increased with the increase of various treatment times,indicating that the plant damage was more serious with the longer the stress time.Research shows cabbage could resist the accumulation of reactive oxygen species in the early stage of stress by activating the antioxidant system,and when plants were unable to resist stress,the dynamic imbalance of reactive oxygen species led to biofilm damage.
作者
赵琳琳
杨旭妍
葛雯冬
王玉书
ZHAO Linlin;YANG Xuyan;GE Wendong;WANG Yushu(School of Life Scinences,Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China)
出处
《高师理科学刊》
2024年第9期59-63,80,共6页
Journal of Science of Teachers'College and University
基金
国家自然科学基金项目(31401908)
黑龙江省自然科学基金项目(C2016056)
黑龙江省省属高等学校基本科研业务费科研项目(145209513,145209319,135509702)
黑龙江省普通高等学校青年创新人才培养计划(UNPYSCT-2017155)
黑龙江省高等教育教学改革研究与实践项目(SJGY20220406)。