香茅草种质资源功能性分析评价

Functional Analysis and Evaluation of Cymbopogon citratus Germplasm Resources

通过对香茅草(Cymbopogon citratus)的甜菜碱、生物碱、黄酮含量的差异分析,进行种质资源的功能性分析评价。本研究收集海南、福建、贵州和云南等地的45份香茅草资源,分别测定水提物的甜菜碱、生物碱、黄酮含量。研究结果表明:香茅草水提物主要包含黄酮类化合物(24.30%)、生物碱类化合物(13.53%)和甜菜碱单体(10.52%)。香茅草HK-2的甜菜碱含量最高,达5.65 mg/g;HK-1-1的生物碱含量最高,达8.66 mg/g;YX-1的黄酮含量最高,达43.15 mg/g。不同保存地、不同地区、不同海拔香茅草的甜菜碱、生物碱、黄酮平均含量差异显著。采用洛哌丁胺诱导的便秘小鼠模型证明甜菜碱具有润肠通便功效。最后,根据建立的香茅草功能性资源评价方法,筛选得到一级香茅草资源YX-1、WN-2、GM-1、ZZ-1、RL-1、YJ-4。本研究结果有助于香茅草功能性资源的筛选评价与育种利用,也可为功能性作用机制研究及功能性产品开发利用提供科学依据。

To evaluate the functional germplasm resources of Cymbopogon citratus,the differences in betaine,alkaloid and flavone contents were analyzed.45 C.citratus from Hainan,Fujian,Guizhou and Yunnan were evaluated by analyzing the content of betaine,alkaloids and flavonoids.The water extract of C.citratus mainly contained flavonoids(24.30%),alkaloids(13.53%)and betaine monomers(10.52%).The betaine content of HK-2 was the highest,reaching 5.65 mg/g.The alkaloid content of HK-1-1 was the highest,reaching 8.66 mg/g.The flavonoid content of YX-1 was the highest,reaching 43.15 mg/g.There were significant differences in the content of betaine,alkaloid and flavonoid in different storage locations,regions and altitudes of the resources.The mouse model of constipation induced by loperamide demonstrated that betaine had the effect of moistening the intestines and promoting bowel movements.Finally,based on the established functional resource evaluation method for C.citratus,first-class C.citratus resources YX-1,WN-2,GM-1,ZZ-1,RL-1 and YJ-4 were selected.The results of this study are helpful for the screening,evaluation and breeding utilization of functional resources of C.citratus,and could also provide scientific basis for the study of functional mechanisms and the development and utilization of functional products.