金线莲多糖通过抑制NF-κB信号通路对糖尿病小鼠肾损伤的改善作用

Improvement Effects of Anoectochilus roxburghii Polysaccharose on Renal Injury in Diabetic Mice by Inhibiting NF-кB Signaling Pathway

为了考察金线莲多糖对糖尿病小鼠肾损伤的改善作用,将糖尿病小鼠随机分为模型组、二甲双胍组及高、低剂量金线莲多糖组(100和300 mg·kg^(-1)),给药4周后测定小鼠血清生化指标、炎症因子及肾皮质中的蛋白表达,并观察肾组织形态学变化。结果表明:与正常组比,模型组小鼠24 h的尿蛋白质量,血肌酐浓度、丙二醛浓度、炎症因子显著升高,而血清超氧化物歧化酶活力显著下降;模型组小鼠肾组织p-NF-κB p65的蛋白表达显著上调,podocin,Nephrin的蛋白表达明显下调;组织学检查显示模型组小鼠肾组织结构异常改变;经金线莲多糖干预后,糖尿病小鼠血清生化指标、肾组织中蛋白表达均明显逆转,组织学检查也显示糖尿病小鼠肾损伤明显改善。

To investigate the improvement effect of Anoectochilus roxburghii polysaccharide on renal injury in diabetic mice,the diabetic mice were randomly divided into model group,metformin group and Anoectochilus roxburghii polysaccharide groups(100 and 300 mg·kg^(-1)).After 4 weeks of treatment,the serum biochemical indexes,inflammatory factors and protein expression in renal cortex of the mice were measured,and the renal morphological changes were observed.The results showed that compared with the control group,24-hour urine protein mass,serum creatinine concentration,malondialdehyde content and inflammatory factors of mice in model group were significantly increased,while serum superoxide dismutase activity was significantly decreased.The protein expression of p-NF-κB p65 in renal tissue of model group was significantly up-regulated,and the protein expressions of podocin and Nephrin were significantly down-regulated.Histological examination showed abnormal changes of renal tissue structure of mice in model group.The serum biochemical indexes and protein expression in kidney tissue of diabetic mice were significantly reversed after the intervention of the Anoectochilus roxburghii polysaccharide,and the histological examination also showed that the kidney injury of diabetic mice treated with Anoectochilus roxburghii polysaccharide was significantly improved.