鹦鹉热衣原体肺炎患者巨噬细胞功能变化及极化水平

Change of function and polarization levels of macrophages in patients with Chlamydia psittaci pneumonia

目的探讨鹦鹉热衣原体感染对巨噬细胞功能变化及极化水平的影响。方法收集2020年3月-2023年2月在福建医科大学附属闽东医院就诊的鹦鹉热衣原体肺炎患者32例为肺炎组,另选同期健康体检者30名为健康组,检测全部研究对象呼吸道深部分泌物巨噬细胞占比、胞饮功能,流式细胞术检测巨噬细胞表面标志因子CD86、CD206的表达,以及M1型巨噬细胞极化标志物白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、单核细胞趋化蛋白-1(MCP-1)mRNA与蛋白活化水平,M2型巨噬细胞极化标志物IL-10、转化生长因子-β(TGF-β)mRNA与蛋白活化水平和自噬相关标志物Beclin-1、LC3-I及LC3-II mRNA转录与蛋白活化水平。结果与健康组相比,肺炎组白细胞计数(12.34±1.49)×10^(5)个/ml、巨噬细胞占比(64.87±2.79)%升高(均P<0.05),OD值降低(P<0.05);与健康组相比,肺炎组巨噬细胞表面标志因子CD86、CD206表达升高(均P<0.05);巨噬细胞M1型极化标志物IL-1β、TNF-α、MCP-1以及M2型极化标志物IL-10、TGF-β的mRNA转录水平及蛋白相对表达水平升高(均P<0.05);肺炎组自噬相关受体Beclin-1、LC3-I、LC3-II的mRNA转录水平及蛋白相对表达水平升高(均P<0.05)。结论鹦鹉热衣原体可降低巨噬细胞胞吞功能,并促进巨噬细胞极化,其中M1型巨噬细胞极化水平高于M2。

OBJECTIVE To explore the change of function and polarization levels of macrophages in the patients with Chlamydia psittaci pneumonia.METHODS A total of 32 patients with C.psittaci pneumonia who were treated in Mindong Hospital Affiliated to Fujian Medical University from Mar 2020 to Feb 2023 were assigned as the pneumonia group,meanwhile,30 healthy people who received physical examination were chosen as the healthy group.The percentage of macrophages in deep respiratory tract secretions and pinocytosis function were detected for all the enrolled research subjects.The expressions of surface markers of macrophages CD86 and CD206 were detected by flow cytometry,and the levels of M1 macrophage polarization markers interleikin-1β(IL-1β),tumor necrosis factor-α(TNF-α),monocyte chemoattractant protein(MCP-1)mRNA and protein activation were also detected;the levels of M2 macrophage polarization markers IL-10,transforming growth factor beta(TGF-β)mRNA and protein activation as well as the levels of autophagy-related markers Beclin-1,LC3-I and LC3-II mRNA transcription were detected.RESULTS The percentages of white blood cells and macrophages of the pneumonia group were respectively(12.34±1.49)×10^(5)/ml and(64.87±2.79)%,higher than those of the healthy group(all P<0.05),while the OD value of the pneumonia group was lower than that of the healthy group(P<0.05).The expression levels of macrophage surface markers CD86 and CD206 of the pneumonia group were higher than those of the healthy group(all P<0.05).The transcription levels of M1 macrophage polarization markers IL-1β,TNF-α,MCP-1 mRNA and M2 polarization markers IL-10 and TGF-βmRNA as well as the relative expression levels of proteins were elevated(all P<0.05).The transcription levels of autophagy-related receptors Beclin-1,LC3-I and LC3-II mRNA and the relative expression levels of proteins of the pneumonia group were higher than those of the healthy group(all P<0.05).CONCLUSION C.psittaci may reduce the endocytosis of the macrophages and promote the polarization.The polarization level of the M1 macrophage is higher than that of the M2 macrophage.