不同基质松节油膏对骨性关节炎大鼠的疗效及关节炎症的影响

Effect of Songjie ointment prepared from different substrates on osteoarthritis(OA)rats and joint inflammation

目的比较以羊油和酥油为基质制作的松节油膏和以凡士林为基质制作的松节油膏对骨性关节炎(Osteoarthritis,OA)大鼠的疗效及关节炎症的影响。方法选取SD雄性大鼠90只为研究对象,10只作为空白组,其余80只大鼠关节注射单碘乙酸钠(Monoiodoacetate,MIA)建立OA模型,造模成功后,随机分为模型对照组(不用任何药物)、阳性对照组(外用消肿止痛酊0.5 mL/次)、松节油膏高剂量组(80 mg/kg)、松节油膏中剂量组(40 mg/kg)、松节油膏低剂量组(20 mg/kg)、凡士林高剂量组(80 mg/kg)、凡士林中剂量组(40 mg/kg)、凡士林低剂量组(20 mg/kg),各给药组3次/d,连续7 w。各组大鼠给药50 d后断尾取血,比较各组大鼠关节炎指数、白细胞介素-1β(Interleukin-1β,IL-1β)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、干扰素-γ(Interferon-γ,IFN-γ)的水平、白细胞(White blood count,WBC)中性粒细胞(Neutrophils,NEU)计数。结果与空白组比较,模型对照组大鼠关节指数,血清IL-1β、TNF-α、IFN-γ水平,WBC及NEU计数均升高(P<0.05);与模型对照组比较,松节油膏高、中、低剂量组和凡士林高、中、低剂量组大鼠关节指数、大鼠血清IL-1β、TNF-α、IFN-γ、WBC及NEU计数显著降低(P<0.05);与松节油膏中剂量组比较,凡士林高中低剂量组大鼠关节指数、大鼠血清IL-1β、TNF-α、IFN-γ水平相对较高(P<0.05)。结论以羊油和酥油为基质制作的松节油膏和以凡士林为基质制作的松节油膏均能改善关节功能,减轻炎症反应和软骨破坏,尤以羊油和酥油为基质制作的松节油膏效果更明显。

Objective To compare the curative effect of turpentine ointment made of sheep oil and ghee and vaseline on osteoarthritis(OA)rats and the effect of joint inflammation.Methods A total of 80 male SD rats were selected for the study,with 10 serving as blank group.The remaining 70 rats were induced with monoiodoacetate(MIA)into their joints to establish an OA model via joint injection.After successful model establishment,the rats were randomly divided into different groups including a model group(no drugs),a positive control group(0.5 mL/time Xiaozhongzhitong),high-dose Songjie ointment groups using sheep oil/ghee as bases(80 mg/kg),medium-dose Songjie ointment groups(40 mg/kg),low-dose Songjie ointment groups(20 mg/kg),high-dose vaseline group(80 mg/kg),medium-dose vaseline group(40 mg/kg),and low-dose vaseline group(20 mg/kg).Each treatment group received medication 3 times per day for 7 consecutive weeks.After 50 days of drug administration,joint inflammation index scores were compared among each group;levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)in serum were measured,white blood cell(WBC)and neutrophil(NEU)counts in serum.Results Compared with control group,the joint index,the serum levels of IL-1β,TNF-αand IFN-γ,the Counts of WBC and NEU in the model control group were increased(P<0.05).The joint index,Serum IL-1β,TNF-α,IFN-γ,WBC and Neu count in high,middle and low dose groups of turpentine ointment and high,middle and low dose groups of Vaseline were significantly decreased(P<0.05).Compared with middle-dose group,the joint index and the serum levels of IL-1β,TNF-αand IFN-γin high-dose,middle-dose and low-dose group of vaseline were higher(P<0.05).Conclusion Turpentine paste made of sheep oil and ghee as the base and vaseline as the base can improve joint function,reduce inflammation and cartilage destruction,especially turpentine paste made of sheep oil and ghee as the base.