仙茅苷调节lncRNA KCNQ1OT1/miR-214-5p轴对骨质疏松大鼠骨代谢的影响王勤俭张攀张睿昕李泊泊

Effect of curculigoside on bone metabolism through regulation of lncRNA KCNQ1OT1/miR-214-5p axis in osteoporotic rats

目的探讨仙茅苷调节长链非编码RNA(lncRNA)KCNQ1重叠转录物1(KCNQ1OT1)/miR-214-5p轴对骨质疏松大鼠骨代谢的改善作用。方法SD大鼠分为对照组、模型组、仙茅苷组、pcDNA组、pcDNA-KCNQ1OT1组、仙茅苷+sh-NC组、仙茅苷+sh-KCNQ1OT1组。ELISA法检测血清中骨钙素(OCN)、碱性磷酸酶(ALP)、肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)水平;三点弯曲实验检测股骨最大位移、最大载荷;Micro-CT检测骨微结构变化;HE染色检测股骨病理变化;qRT-PCR检测股骨中lncRNA KCNQ1OT1、miR-214-5p表达;验证lncRNA KCNQ1OT1与miR-214-5p的关系。结果与对照组比较,模型组骨小梁结构紊乱,OCN、ALP水平、股骨最大位移、最大载荷、骨密度、骨小梁厚度、骨小梁数量、骨体积分数及lncRNA KCNQ1OT1表达降低,TNF-α、IL-6水平、miR-214-5p表达升高(P<0.05);与模型组比较,仙茅苷组骨小梁排列间隙变小,OCN、ALP水平、股骨最大位移、最大载荷、骨密度、骨小梁厚度、骨小梁数量、骨体积分数及lncRNA KCNQ1OT1表达升高,TNF-α、IL-6水平、miR-214-5p表达降低(P<0.05);与pcDNA组比较,pcDNA-KCNQ1OT1组股骨组织病理损伤有所缓解,OCN、ALP水平、股骨最大位移、最大载荷、骨密度、骨小梁厚度、骨小梁数量、骨体积分数及lncRNA KCNQ1OT1表达升高,TNF-α、IL-6水平、miR-214-5p表达降低(P<0.05);sh-KCNQ1OT1逆转了仙茅苷对骨质疏松症大鼠炎症反应及骨生物力学性能的改善作用以及成骨促进作用;lncRNA KCNQ1OT1靶向调控miR-214-5p。结论仙茅苷可能通过调控lncRNA KCNQ1OT1/miR-214-5p轴改善骨质疏松症大鼠骨代谢。

Objective To investigate the improvement effect of curculigoside on bone metabolism in osteoporosis rats by regulating the long non-coding RNA(lncRNA)KCNQ1 opposite strand/antisense transcript 1(KCNQ1OT1)/miR-214-5p axis.Methods SD rats were randomly divided into control group,model group,curculigoside group,pcDNA group,pcDNA-KCNQ1OT1 group,curculigoside+sh-NC group,and curculigoside+sh-KCNQ1OT1 group.ELISA method was applied to detect the serum levels of osteocalcin(OCN),alkaline phosphatase(ALP),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6).Three-point bending experiment was applied to detect the maximum displacement and maximum load of the femur.Micro-CT was applied to detect changes in bone microstructure.HE staining was applied to detect pathological changes in the femoral tissue.qRT-PCR was applied to detect the expressions of lncRNA KCNQ1OT1 and miR-214-5p in femurs.The relationship between lncRNA KCNQ1OT1 and miR-214-5p was verified.Results Compared to those in the control group,the bone trabecular structure in model group was disordered,the levels of OCN and ALP,the maximum displacement of the femur,maximum load,bone mineral density,trabecular thickness,number of trabeculae,bone volume fraction,and the expression of lncRNA KCNQ1OT1 decreased.Levels of TNF-αand IL-6 and the expression of miR-214-5p increased(P<0.05).Compared to those in the model group,the gap between the arrangement of bone trabeculae in the curculigoside group was smaller,the levels of OCN and ALP,the maximum displacement of the femur,maximum load,bone mineral density,trabecular thickness,number of trabeculae,bone volume fraction,and the expression of lncRNA KCNQ1OT1 increased.Levels of TNF-αand IL-6 and the expression of miR-214-5p decreased(P<0.05).Compared to those in the pcDNA group,the pathological damage of the femoral tissue in the pcDNA-KCNQ1OT1 group was alleviated,the levels of OCN and ALP,the maximum displacement of the femur,maximum load,bone mineral density,trabecular thickness,number of trabeculae,bone volume fraction,and the expression of lncRNA KCNQ1OT1 increased.Levels of TNF-αand IL-6 and the expression of miR-214-5p decreased(P<0.05).sh-KCNQ1OT1 reversed the improvement of the inflammatory response and biomechanical properties of the bone and the promoting effect of bone formation in osteoporosis rats.LncRNA KCNQ1OT1 was able to target and to regulate miR-214-5p expression.Conclusion Curculigoside may improve bone metabolism in osteoporosis rats by regulating the lncRNA KCNQ1OT1/miR-214-5p axis.