基于广泛靶向代谢组学的菜心代谢物综合分析

Comprehensive Analysis of Metabolites in Brassica parachinensis Based on Broadly Targeted Metabolomics

对于影响菜心风味和营养品质的代谢物,目前缺乏系统和全面的解析。本研究采用三重四级杆质谱的多反应检测模式,对广东地区4个不同类型的菜心品种的代谢物进行系统解析。在菜心中共检测到519个代谢物,其中210个(40.46%)初生代谢物、288个(55.49%)次生代谢物。D-葡萄糖、蔗糖、脯氨酸、丝氨酸、苏氨酸和组氨酸是菜心甜味的主要贡献物质,天冬氨酸、谷氨酸和赖氨酸是菜心鲜味的主要贡献物质,苹果酸和柠檬酸主要影响菜心的酸味、苦味和鲜味。菜心中含有葫芦巴碱、槲皮素、异鼠李素、木犀草素等多种药用价值较高的次生代谢物。本研究采用广泛的靶向代谢组学分析方法,对菜心的代谢产物进行系统研究,为提高菜心的营养品质和口感风味提供了理论参考。

There is a lack of systematic and comprehensive analysis of the metabolites that affect the flavor and nutritional quality of Brassica parachinensis.In this study,a triple quadrupole mass spectrometry with multiple reaction detection mode was used to systematically resolve the metabolites in four different types of B.parachinensis from the Guangdong region.A total of 519 metabolites,210(40.46%)primary metabolites,288(55.49%)secondary metabolites,and 21(4.05%)belonged to other categories were detected in B.parachinensis.This study concluded that D-glucose,sucrose,proline,serine,threonine and histidine were the main contributors to the sweetness,aspartic acid,glutamic acid and lysine were the main contributors to the umami,and malic acid and citric acid mainly affected the sourness,bitterness and umami of B.parachinensis.In addition,we also found that the B.parachinensis contained a variety of secondary metabolites with high medicinal value,such as trigonelline,quercetin,isorhamnetin,and luteolin.In this study,a comprehensive targeted metabolomic analysis method was utilized to systematically investigate the metabolic products of B.parachinensis,providing important theoretical references for improving its nutritional quality and taste flavor.