千年桐雌雄植株花期生理生化差异分析

Analysis of Physiological and Biochemical Differences Between Female and Male Plants of Vernicia montana at Flowering Stage

为揭示千年桐雌雄植株之间的差异,分别以处在花期的雌雄植株叶片为试材,采用乙醇浸提法、蒽酮比色法、福林试剂测定法、氮蓝四唑法、愈创木酚法、酶联免疫吸附法,比较测定叶片叶绿素、可溶性糖、总酚含量,超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性以及内源激素含量差异。结果表明,千年桐植株叶片叶绿素a、叶绿素b、叶绿素总量、可溶性糖、总酚的含量均表现为雌株高于雄株,但差异不显著(P>0.05)。雄株叶片过氧化物酶(POD)活性、ZR和IAA含量均显著高于雌株(P<0.05),这几个指标与千年桐性别关系密切。

In order to reveal the differences between female and male plants of Vernicia montana,the leaves of V.montana at flowering stage were selected as samples,and ethanol extraction,anthrone colorimetric method,folin reagent assay,nitrogen blue tetrazole method,guaiacol method,and enzyme-linked immuno-sorbent assays were used to determine the contents of chlorophyll,soluble sugar,total phenol,superoxide dismutase(SOD)activity,peroxidase(POD)activity and endogenous hormones in the leaves of female and male plants at flowering stage.The results showed that the contents of chlorophyll a,chlorophyll b,total chlorophyll,soluble sugar and total phenol in the leaves of the female plants were higher than those of the male plants,but the difference was not significant(P>0.05).The peroxidase(POD)activity,ZR content and IAA content in male leaves were significantly higher than those in female leaves plants(P<0.05),which were closely related to the sex of V.montana.