黄精转录组SSR分子标记开发及种质遗传多样性分析

Polygonatum sp.Transcriptome:SSR Markers Development and Genetic Diversity Analysis

为丰富可用于黄精属鉴定的SSR分子标记,开发可用于黄精属(Polygonatum sp.)种质资源鉴定与遗传多样性分析的简单重复序列(simple sequence repeat,SSR)分子标记,为药材黄精正品和伪品的鉴定及遗传多样性分析提供基础。通过对3种黄精属植物进行转录组测序,从60836967个Unigenes鉴定出19630个包含1~6个碱基重复类型的SSR位点,设计和筛选获得了9对SSR有效性引物,并对来源于5个省份共计31份种质资源进行鉴定与遗传多样性分析。9对SSR引物不仅能够将长梗黄精与其他3种药材黄精划分开,还能够较好地区分不同基源黄精(滇黄精、黄精、多花黄精),并且31份资源遗传相似系数范围为0.0769~0.75。此外,构建了31份黄精属种质资源的DNA指纹图谱数据库,开发了相对应的指纹图谱。本研究结果为黄精属种质资源的分类鉴定、遗传多样性分析和品种选育提供了可用的分子标记以及参考依据。

To develop simple sequence repeat(SSR)markers for germplasm resource identification and genetic diversity analysis in Polygonatum sp.,transcriptome sequencing was conducted on 3 species of Polygonatum sp.,from which 19630 SSR loci containing 1-6 base repeats were identified out of 60836967 Unigenes.9 pairs of effective SSR primers were designed and screened,and 31 Polygonatum germplasm resources from 5 provinces were identified and subjected to genetic diversity analysis.The 9 pairs of SSR primers could not only distinguish P.filipes from other species of Polygonatum,but also effectively differentiate between different sources of Polygonatum(P.sibiricum,P.kingianum,and P.cyrtonema),with the genetic similarity coefficient of the 31 resources ranging from 0.0769 to 0.75.Additionally,a DNA fingerprint database for 31 germplasm resources of Polygonatum sp.was established,along with the development of the corresponding fingerprint maps.The results of this study provide useful molecular markers and a reference basis for the classification and identification,genetic diversity analysis,and variety selection of Polygonatum germplasm resources.