经典名方当归补血汤中挥发性成分量值传递规律研究

Quantitative value transfer pattern of volatile components in classic formula Danggui Buxue Decoction

目的通过气相色谱-质谱(GC-MS)技术对经典名方当归补血汤在饮片、基准样品、提取液、浓缩液、浸膏粉及颗粒中的挥发性成分进行检测,建立多指标成分含量测定方法,探究其量值传递规律。方法采用GC-MS技术对当归补血汤基准样品中挥发性成分进行指认,判定其成分归属,借助NIST 20s.lib数据库及对照品比对筛选出共有成分,建立共有成分的GC-MS含量测定方法,探究其在饮片-基准样品、饮片-提取液、提取液-浓缩液、浓缩液-浸膏粉、浸膏粉-颗粒中的量值传递规律。结果当归补血汤基准样品中共检出10个响应较好的成分,其中2个来自黄芪,8个来自酒当归,筛选出苯甲酸、2-甲氧基-4-乙烯基苯酚、藁本内酯、洋川芎内酯H 4个共有成分。含量测定结果表明此4个成分从饮片到颗粒得到有效传递,且苯甲酸、2-甲氧基-4-乙烯基苯酚和洋川芎内酯H在颗粒的转移率与基准样品较接近,而藁本内酯在浓缩和喷干工艺中损失较多,导致转移率相差较大。结论从饮片到颗粒的传递过程中,不同挥发性成分的传递规律不一致,有必要优选合适生产工艺保留挥发性成分,并选择合理评价指标进行质量控制。

Objective The volatile components of the classic formula Danggui Buxue Decoction were detected by GC-MS in tablets,baseline samples,extract powder,concentrates,extracts and granules,to establish a multi-indicator method for the determination of the content of the components and to investigate the transmission law of the quantitative value.Method The volatile components in the baseline sample of Danggui Buxue Decoction were identified by GC-MS,and the attribution of the components was determined.With the help of NIST 2os.lib database and standard comparison,the common components were screened out,and the method for the determination of the content of the common components by GC-MS was established,and the quantitative value transfer patterns were investigated in the tablets-baseline samples,tablets-extracts,extracts-concentrates,concentrates-extract powder,extract powdergranules.Results In the baseline sample of Danggui Buxue Decoction,a total of 10 components were detected,among which two components were from Astragalus membranaceus(Fisch.)Bunge and eight components were from Angelica sinensis,and four common components,including benzoic acid,2-methoxy-4-vinylphenol,ligustilide,and senkynolide H,were screened out.The results showed that these four components were effectively transferred from tablets to granules,and the transfer rates of benzoic acid,2-methoxy-4-vinylphenol and senkynolide H to granules were close to those of the baseline samples,whereas ligustilide was more lost in the concentration and spray-drying processes,resulting in a larger difference in the transfer rates.Conclusion The transfer pattern of different volatile components from slices to granules was inconsistent,and it is necessary to prefer suitable production processes to retain the volatile components and select reasonable evaluation indexes for quality control.