山豆根菌核病病原菌鉴定及室内药剂筛选

Identification of pathogen of sclerotinia on Sophora tonkinensis Radix and screening of laboratory agents

采用常规组织分离法分离山豆根(Sophora tonkinensis Radix)菌核病病原菌,利用柯赫式法则进行致病性测定,基于形态特征、菌丝融合群测定、细胞核荧光染色以及rDNA-ITS序列分析进行病原菌的鉴定,并利用菌丝生长速率法测定5种杀菌剂对病原菌的抑制活性。结果表明,山豆根菌核病的病原菌为立枯丝核菌(Rhizoctonia solani),属于融合群AG-1。98%恶霉灵可溶性粉剂(SP)、75%肟菌·戊唑醇水分散粒剂(WG)、250 g/L吡唑醚菌酯乳油(EC)、10亿/g哈茨木霉菌悬浮剂(SC)、50%多菌灵可湿性粉剂(WP)对病原菌菌丝的生长均有较好的抑制效果,抑制中浓度(EC_(50))分别为0.0611、0.5757、0.0748、0.2843、0.7405 mg/L。

The pathogens from Sophora tonkinensis Radix were isolated by conventional tissue separation method,the pathogenicity was determined by Koch’s formula,and the pathogens were identified based on morphological characteristics,mycelium fusion group determination,nuclear fluorescence staining and rDNA-ITS sequence analysis.The inhibitory activities of 5 fungicides against patho-gens were measured by the mycelium growth rate method.The results showed that the pathogen was Rhizoctonia solani,belonging to the fusion group AG-1.98%hymexazol soluble power(SP),75%oxime·pentazolol water dispersible granule(WG),250 g/L pyra-clostrobin emulsifiable concentrate(EC),1 billion/g Trichoderma harziana suspension concentrate(SC),and 50%carbendazim wet-table power(WP)all had good inhibitory effects on the growth of pathogenic fungi mycelia.The inhibitory medium effective concentra-tions(EC_(50))were 0.0611,0.5757,0.0748,0.2843,0.7405 mg/L,respectively.