多倍体水稻DHF-CBS基因的抗旱性鉴定

Identification of drought resistance of polyploid Oryza sativa L.DHF-CBS gene

为探究干旱胁迫下DHF-CBS基因调控多倍体水稻(Oryza sativa L.)的抗旱机制,选取2种水稻品种,分别为多倍体植株中花-11(DHF-CBS基因)、突变体植株UC-32(不表达DHF-CBS基因),对2种水稻的GUS化学染色结果、组织含水量、脱落酸(ABA)含量、硫代巴比妥酸活性物质(TBARS)含量、谷胱甘肽(GSH)含量等进行分析。结果表明,干旱胁迫第7天,中花-11仍有部分叶片保持绿色且坚挺,干旱胁迫第11天时整株叶片变黄,甚至有的叶片变枯,经过干旱胁迫后的表型观察发现,中花-11比UC-32的抗旱能力强;对水稻的含水量、ABA含量、TBARS含量、GSH含量进行分析,表明中花-11的抗旱性优于UC-32,说明DHF-CBS基因增强了水稻的抗旱能力。

In order to explore the drought resistance mechanism of DHF-CBS gene regulation in polyploid Oryza sativa L.under drought stress,two varieties of Oryza sativa L.were selected,namely polyploid plant Zhonghua-11(DHF-CBS gene)and mutant plant UC-32(non expressing DHF-CBS gene),the GUS chemical staining results,tissue moisture content,abscisic acid(ABA)con-tent,thiobarbituric acid reactive substance(TBARS)content,glutathione(GSH)content,etc.of two types of Oryza sativa L.were analyzed.The results showed that on the 7th day of drought stress,some leaves of Zhonghua-11 remained green and firm.On the 11th day of drought stress,the entire plant’s leaves turned yellow,and some leaves even withered,after observing the phenotype under drought stress,it was found that Zhonghua-11 had stronger drought resistance than UC-32.Analysis of the water content,ABA con-tent,TBARS content,and GSH content of Oryza sativa L.showed that the drought resistance of Zhonghua-11 was better than that of UC-32,indicating that the DHF-CBS gene enhanced the drought resistance of Oryza sativa L..