基于液相色谱-三重四级杆质谱仪的栀子及水栀子中萜类物质鉴定及成分差异研究

Identification of and investigation into composition differences of terpenoids in Gardenia jasminoides Ellis and Gardenia jasminoides var.radicans Makino using liquid chromatography triple quadrupole mass spectrometer

目的建立栀子中萜类物质的高通量分析方法,并将其应用于栀子与水栀子的成分差异研究。方法结合液相色谱-三重四级杆质谱仪中的全扫描、子离子扫描、母离子监测、中性丢失离子扫描、多反应离子监测、动态多反应离子监测等多种技术提出栀子中萜类物质的定性、定量分析策略,进一步应用主成分分析和正交偏最小二乘判别分析等多元变量分析方法研究栀子与水栀子的成分差异。结果共鉴定出并相对定量81种萜类物质,发现栀子与水栀子间萜类物质含量差异显著,其中13种物质可作为鉴别栀子与水栀子的差异标志物。结论所建立的方法操作便捷,结果准确,为栀子药材鉴别及质量评价提供了新思路,并为栀子资源的合理开发利用提供了参考依据。

Objective To develop a method for the high-throughput determination of terpenoids in Gardenia jasminoides Ellis and apply it in an investigation of composition differences between Gardenia jasminoides Eills and Gardenia jasminoides var.radicans Makino.Methods A high-throughput qualitation and quantifica-tion strategy was proposed for the terpenoids in Gardenia jasminoides Ellis by combining multiple techniques such as full scanning,product ion scanning,precursor ion monitoring,neutral loss ion scanning,multiple reac-tion monitoring,and dynamic multiple reaction ion monitoring in liquid chromatography-triple quadrupole mass spectrometry,the multivariate analysis methods such as principal component analysis and orthogonal partial least squares-discriminant analysis were further applied for investigating the composition differences.Results A total of 81 terpenoids were identified and relatively quantified in the present study.There was a sig-nificant difference in the content of terpenoids between Gardenia jasminoides Ellis and Gardenia jasminoides var.radicans Makino.Of them,13 different substances could be used as markers for distinguishing Gardenia jasminoides Ellis and Gardenia jasminoides var.radicans Makino.Conclusion The established method is con-venient and accurate,which provides a new idea for the identification and quality evaluation of gardenia,and provides a reference for the rational development and utilization of gardenia resources.