基于多组学探究STIP1在肝细胞癌中的表达特征及临床联系

A multi-omics-based exploration of the expression profile and clinicopathological association of STIP1 in hepatocellular carcinoma

目的基于多组学探究STIP1在肝细胞癌中的功能特征及临床意义。方法基于公共数据库肝细胞癌mRNA表达数据,多维度分析肝细胞癌组织中STIP1的表达及其临床意义;分析STIP1表达与肿瘤免疫细胞浸润丰度、免疫检查点、细胞趋化因子的相关性,基于STIP1表达与临床病理特征构建预后列线图模型。结果STIP1在肝细胞癌中显著上调并且与肝细胞癌的组织学分级、病理分期、T分期、N分期、M分期、体重指数及甲胎蛋白的表达水平及肝纤维化程度显著相关;且STIP1的高表达与患者较差生存预后显著相关;STIP1对肝细胞癌表现出良好的诊断效能;STIP1的表达水平与肝细胞癌中CD4+T细胞、T细胞调节细胞、单核细胞和髓系树突状细胞的浸润丰度及部分免疫检查点表现出显著的相关性;药敏分析提示STIP1与肝细胞癌化疗及靶向治疗药物敏感性相关。结论STIP1可能是肝细胞癌的预后标志物与潜在预测靶点,STIP1表达与肝细胞癌的药物敏感性存在相关性。

Objective To investigate the functional characteristics and clinical significance of stress induced phosphoprotein 1(STIP1)in hepatocellular carcinoma(HCC)based on multi-omics.Methods Based on the mRNA expression data of HCC from the public database,the expression of STIP1 in HCC tissues was ana-lyzed multidimensionally.The correlation between STIP1 and the abundance of tumor immune cell infiltra-tion,immune checkpoints and chemokines was analyzed,and a prognostic nomogram model was constructed based on the expression level of STIP1 and linicopathological characteristics.Results STIP1 was significantly up-regulated in hepatocellular carcinoma and meanningfully correlated with the histologic grade,pathological stage,T stage,N stage,M stage,body mass indes,α-fotoprotein expression level,as well as the degree of liver fibrosis.The high expression of STIP1 was significantly correlated with a poor survival prognosis of patients,and STIP1 showed good diagnostic efficacy for hepatocellular carcinoma.The expression level of STIP1 was significantly correlated with the abundance of CD4+T cells,T cell regulatory cells,monocytes,and infil-trated myeloid dendritic cells as well as with some immune checkpoints in hepatocellular carcinoma.STIP1 also correlated with sensitivity to chemotherapy and targeted therapies in some hepatocellular carcinomas.Conclusion STIP1 may be a prognostic marker and a potential predictive target in hepatocellular carcinoma,and there is a correlation between STIP1 and drug sensitivity in hepatocellular carcinoma.