miR-21-5p抑制TLR4诱导皮肤鳞状细胞癌细胞增殖

miR-21-5p inhibited TLR4-induced cells proliferation of cutaneous squamous cell carcinoma

目的研究TLR4与miR-21-5p对皮肤鳞状细胞癌细胞增殖的影响及两者的靶向关系。方法通过免疫组化法检测皮肤鳞状细胞癌组织中的TLR4表达,通过Toll样受体4(TLR4)质粒和miR-21-5p inhibitor转染过表达细胞中的TLR4和抑制细胞中miR-21-5p,通过生物信息学分析TLR4与miR-21-5p的关系,通过RT-qPCR检测细胞中TLR4和miR-21-5p的表达,通过CCK8检测A431细胞生长情况,通过Western bloting检测细胞中TLR4和增殖分子ki-67的表达。结果TLR4在皮肤鳞状细胞癌癌旁组织中表达最高,且在低分化组织中表达低于高分化组织,差异有统计学意义(P<0.05),TLR4在A431人皮肤鳞状细胞癌细胞中表达低于HaCaT人永生化角质形成细胞,差异有统计学意义(P<0.05)。miR-21-5p可能是TLR4的潜在miRNA靶基因。A431细胞过表达TLR4,发现细胞增殖抑制,差异有统计学意义(P<0.05),miR-21-5p表达降低,差异有统计学意义(P<0.05)。A431细胞抑制miR-21-5p表达,发现细胞增殖抑制,差异有统计学意义(P<0.05),TLR4表达降低,差异有统计学意义(P<0.05)。结论miR-21-5p与TLR4的相互调节影响A431细胞的增殖,miR-21-5p与TLR4的相互作用在皮肤鳞状细胞癌的细胞行为中发挥重要作用。

Objective To investigate the effects of TLR4 and miR-21-5p on the proliferation of cutaneous squamous cell carcinoma and their targeting relationship.Methods TLR4 expression in skin squamous cell carcinoma tissues was detected by immunohistochemistry,TLR4 in overexpressing cells and miR-21-5p in inhibiting cells were transfected by Toll-like receptor 4(TLR4)plasmid and miR-21-5p inhibitor,the relationship between TLR4 and miR-21-5p was analyzed by bioinformatics,and the relationship between TLR4 and miR-21-5p in skin squamous cell carcinoma was detected by RT-qPCR.Expression of TLR4 and miR-21-5p in cells,and the growth of A431 cells by CCK8,and the expression of TLR4 and the proliferation molecule ki-67 in cells by western bloting.Results TLR4 was expressed the highest in adjacent tissues of cutaneous squamous cell carcinoma,and its expression was lower in poorly differentiated tissues than in well differentiated tissues(P<0.05).TLR4 was expressed lower in A431 human cutaneous squamous cell carcinoma cells than in HaCaT human immortalized keratinocytes(P<0.05).A431 cells inhibited the expression of miR-21-5p(P<0.05),and the inhibition of cell proliferation was detected,and the difference was statistically significant(P<0.05),TLR4 expression was reduced,the difference was statistically significant(P<0.05).Conclusion The mutual regulation of miR-21-5p and TLR4 affects the proliferation of A431 cells,and the interaction between miR-21-5p and TLR4 may play an important role in the cellular behavior of cutaneous squamous cell carcinoma.