LncRNA LIPCAR在慢性心力衰竭患者外周血中的表达及临床意义

The expression and clinical significance of LncRNA LIPCAR in peripheral blood of patients with chronic heart failure

目的证实长链非编码RNA LIPCAR(LncRNA LIPCAR)在慢性心力衰竭患者中的表达,探讨LncRNA LIPCAR的表达水平与心脏左室射血分数(LVEF)、血清中氨基末端脑钠肽前体(NT-proBNP)的相关性及其对心力衰竭诊断的效能。方法选取住院患者共80例作为研究对象,依据左室射血分数值将其分为正常对照组(n=20)、心力衰竭射血分数降低组(n=20)、射血分数保留组(n=20)、射血分数中间值组(n=20),采用荧光定量PCR法检测血浆LncRNA LIPCAR水平,双向测流免疫法检测NT-proBNP含量。应用Pearson线性相关分析查验心衰患者LncRNA LIPCAR水平与LVEF值、NT-proBNP的相关性,根据受试者工作特征(ROC)曲线下面积(AUC)比较LncRNA LIPCAR联合NT-proBNP水平、LVEF值对心力衰竭的预测价值。结果慢性心力衰竭患者LVEF值低于正常对照组(P<0.05);心力衰竭射血分数降低组、射血分数中间值组患者LncRNA LIPCAR值明显高于正常对照组(P<0.05)。慢性心力衰竭患者LncRNA LIPCAR水平与LVEF值呈负相关(P<0.05),与NT-proBNP水平呈正相关(P<0.05)。LncRNA LIPCAR、NT-proBNP水平、LVEF值单独及三者联合预测慢性心衰患者的ROC曲线下面积(AUC)分别为0.910、0.989、0.847和1.000。结论LncRNA LIPCAR在射血分数降低及中间值心衰患者中异常表达,联合NT-proBNP水平、LVEF值对心力衰竭诊断及预后的预测价值更高。

Objective To study the expression of LncRNA LIPCAR in patients with chronic heart failure,and to explore the correlation between the expression level of LncRNA LIPCAR and left ventricular ejection fraction(LVEF),N-terminal proBNP precursor(NT proBNP)in serum,and its diagnostic efficacy in heart failure.Method A total of 80 inpatients were selected as study subjects based on the left ventricular ejection fraction values were divided into control group(n=20),heart failure ejection fraction reduction group(n=20),ejection fraction retention group(n=20)and ejection fraction median group(n=20).The levels of plasma LncRNA LIPCAR were detected by fluorescence quantitative PCR,and the level of NT proBNP were detected by double directimal flow immunoqssay.Pearson linear correlation analysis was applied to examine the correlation between the levels of LncRNA LIPCAR,LVEF values and NT proBNP in heart failure patients and the predictive value of LncRNA LIPCAR combined with NT-proBNP and LVEF values for heart failure was compared according to the area under the curve(AUC)of the working characteristics(ROC)of the subjects.Results The LVEF value in patients with chronic heart failure were lower than those of the normal group(P<0.05).The levels of LncRNA LIPCAR in the group with decreased ejection fraction and in the group with intermediate ejection fraction in heart failure was significantly higher than those in the normal group(P<0.05).The level of LncRNA LIPCAR in patients with chronic heart failure was negatively correlated with LVEF values(P<0.05)and positively correlated with NT-proBNP(P<0.05).The area under the ROC curve(AUC)of LncRNA LIPCAR,NT-proBNP,and LVEF values alone and in combination of all three predicted the area under the ROC curve(AUC)for patients with chronic heart failure were respectively 0.910,0.989,0.847 and 1.000.Conclusion LncRNA LIPCAR is abnormally expressed in patients with decreased ejection fraction and median heart failure.The combination of NT proBNP and LVEF values has higher predictive value for the diagnosis and prognosis of heart failure.