PBK促进前列腺癌患者放疗抵抗

PBK promotes radiotherapy resistance in prostate cancer patients

目的:探索PDZ结合激酶(PDZ-binding-kinase,PBK)在前列腺癌放射治疗中的影响和预后意义。方法:从基因表达综合(gene expression omnibus,GEO)数据库中下载寻找前列腺癌细胞中放疗抵抗基因,选取符合阈值的样本资料。随后分析样本中前列腺癌组织与正常组织中PBK的表达差异。基于PBK的表达绘制前列腺癌的生存曲线,利用Western Blot检测不同放疗剂量下PBK的表达情况,最后收集50例局部晚期前列腺癌患者,分析PBK表达与放疗敏感性的关系。结果:PBK是前列腺癌的放疗抵抗基因,PBK在肿瘤细胞中高表达,PBK高表达患者总生存率低。当放疗剂量提高时,PBK的表达也相应增高,放疗抵抗患者PBK表达高于放疗敏感者。结论:PBK促进前列腺癌患者放疗抵抗,并且有可能成为前列腺癌的潜在治疗靶点。

Objective:To investigate the effect and prognostic significance of PDZ-binding-kinase(PBK)in radiotherapy for prostate cancer.Methods:The radiotherapy resistance genes in prostate cancer cells were downloaded from the gene expression omnibus(GEO)database,and the samples meeting the threshold were selected.The differences in PBK expression between prostate cancer tissues and normal tissues were analyzed.The survival curve of prostate cancer was drawn based on the expression of PBK.Finally,Western Blot was used to detect the expression of PBK under different radiotherapy doses.Results:PBK was the radiotherapy resistance gene of prostate cancer,and PBK was highly expressed in tumor cells.In patients with high PBK expression,the former overall survivals was lower.When the radiotherapy dose was increased,the expression of PBK was also increased.Conclusion:PBK can promote radiotherapy resistance in patients with prostate cancer,and may be a potential therapeutic target for prostate cancer.