高效液相色谱内标法测定海水叶绿素a浓度

Determination of Chlorophyll a Concentration in Seawater by High Performance Liquid Chromatography Using Internal Standard Method

叶绿素a浓度是海水水质监测和海洋赤潮预警的重要指标,高效液相色谱法作为叶绿素a浓度最准确的测量方法之一被广泛应用。本文选用β-阿朴-8’-胡萝卜素醛(β-Apocarotena-8’-carotenal,Apocarotenal)为内标物,建立了一种基于液相色谱内标法的海水叶绿素a浓度分析方法,在实验检测范围内,方法相关性良好,相关系数为0.9998,检出限为0.05μg/L,回收率为97.0%~100%,相对标准偏差为0.38%~0.55%,精密度良好。同时,利用实验室培养的小球藻藻液和现场海水样品对内标法和外标法进行了显著性差异评估。结果表明:两种方法不存在显著性差异,12组现场海水样品内标法和外标法的测定结果相关性良好,相关系数为0.92,平均相对误差为6.21%。液相色谱内标法测定海水叶绿素a浓度分离效果好、准确度高,能对实验室叶绿素a浓度的测量进行较为准确的质量控制。

Chlorophyll a concentration is an important index of seawater quality monitoring and early warning of Marine red tide.High per-formance liquid chromatography is widely used as one of the most accurate measurement methods of Chlorophyll a concentration.茁-Apoc-arotenal-8爷-carotenal was selected as internal standard to establish a method for the concentration analysis of Chlorophyll a in seawater based on high performance liquid chromatographic internal standard method.In the experimental detection range,the correlation coeffi-cient was 0.9998,the detection limit was 0.05滋g/L,and the recovery was 97.0%-100%.The relative standard deviation was 0.38%-0.55%,and the precision was good.At the same time,the significant difference between the internal and external standard methods was e-valuated by using the laboratory culture of chlorella algal fluid and the field seawater samples,and the results showed that there was no significant difference between the two methods.The results of internal standard method and external standard method in 12 groups of field seawater samples showed good correlation,the correlation coefficient was 0.92,and the average relative error was 6.21%.HPLC internal standard method for the determination of Chlorophyll a concentration in seawater has good separation effect and high accuracy,and can carry out more accurate quality control for the measurement of Chlorophyll a concentration in laboratory.