贵州废弃烟叶中具有α-糖苷酶抑制活性的多酚类化合物的分离纯化与结构鉴定

Isolation,Purification and Structural Identification of Polyphenolic Compounds withα-glucosidase Inhibitory Activity from Guizhou Discarded Tobacco Leaves

为了探究贵州废弃烟叶在降低血糖方面的物质基础,以贵州废弃烟叶为原料,通过提取、浓缩、硅胶柱层析、凝胶柱层析等技术方法从其乙醇提取物的石油醚中分离纯化获得9个多酚类单体化学成分,通过ESI-MS、^(1)H-NMR、^(13)C-NMR、DEPT等现代波谱学检测解析技术对所有得到的化合物进行了结构鉴定,被鉴定的9个多酚类单体化合物分别为Chlorogenicacid(1)、Rutin(2)、5,7,4'-trihydroxy-3,3'-dimethoxyflavone(3)、5,7-dihydroxyl-6,8,4'-trimethoxylflavone(4)、Kaempferol-3-methyl ether(5)、Scosoletin(6)、Dihydroferulic acid methyl ester(7)、2-(4-hydroxyphenyl)ethanol(8)、1,2,4,5-tetrahydroxybenzene(9)。继续采用PNPG法测定多酚类单体化合物的α-糖苷酶抑制活性,结果显示化合物9具有显著的抑制α-糖苷酶的活性,且优于阳性药阿卡波糖,这表明化合物9具有成为α-糖苷酶抑制剂的潜力,也为进一步探讨贵州废弃烟叶多用途和研制天然α-糖苷酶抑制剂产品奠定了科学依据。

The polyphenols in the ethanol extract of waste tobacco leaves from Guizhou were prepared by extraction,concentration and column chromatography.The structures of all the obtained compounds were identified by ESI-MS,^(1)H-NMR,^(13)C-NMR,DEPT and other modern spectroscopic techniques.Nine polyphenols were identified as Chlorogenic acid(1),Rutin(2),5,7,4-trihydroxy-3,3-dimethoxyflavone(3),5,7-dihydroxyl-6,8,4-trimethoxylflavone(4),Kaempferol-3-methyl ether(5),Scosoletin(6),Dihydroferulic acid methyl ester(7),2-(4-hydroxyphenyl)ethanol(8),1,2,4,5-tetrahydroxybenzene(9).Theα-glucosidase inhibitory activity test showed that compound 9 had betterα-glucosidase inhibitory activity than the positive drug acarbose,indicating that compound 9 had the potential to become anα-glucosidase inhibitor.It also provided a certain research basis for further exploring multi-purpose waste tobacco leaves and developing naturalα-glucosidase inhibitor products.