高效液相色谱法测定乳制品中泛酸的测量不确定度评定

Evaluation of Measurement Uncertainty for Determination of Pantothenic Acid in Dairy Products with High Performance Liquid Chromatography

目的:评定高效液相色谱法测定乳制品中泛酸的测量不确定度。方法:根据《测量不确定度评定与表示》(JJF 1059.1—2012),采用《食品安全国家标准食品中泛酸的测定》(GB 5009.210—2023)第一法高效液相色谱法,结合不确定度评定,对乳制品中泛酸含量进行测定后建立数学模型,分析并评定不确定度来源。结果:通过对不确定度各分量进行量化和合成,结果显示乳制品样品中泛酸的含量为15.86 mg/100 g,扩展不确定度为0.50 mg/100 g(k=2)。结论:本实验不确定度主要集中在泛酸加标试验回收率、各标准溶液的配制和标准曲线的拟合过程,相比之下,样品的称量及其前处理过程引入的不确定度分量相对最小。

Objective:Evaluation of uncertainty for determination of pantothenic acid in dairy products by high performance liquid chromatography.Method:According to JJF 1059.1-2012,reference to the first method of GB 5009.210-2023,combining uncertainty assessment,established a mathematical model to determine the content of pantothenic acid in dairy products by liquid chromatography,analyzed and evaluated the sources of the uncertainty.Result:By quantifying and synthesizing the various components of uncertainty,the results showed that the content of pantothenic acid in the dairy product sample was 15.86 mg/100 g,and the extended uncertainty was 0.50 mg/100 g(k=2).Conclusion:This experiment showed that the preparation of standard solution and curve fitting has a great influence on the accuracy of the results,and the repeatability determination has a smaller influence on the accuracy of the results.